مدل‌سازی انحلال فسفر توسط Pseudomonas fluorescens با استفاده از روش سطح پاسخ

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری گروه علــوم خاک، دانشکده کشاورزی دانشگاه ارومیه

2 دانشیار گروه علــوم خاک، دانشکده کشاورزی دانشگاه ارومیه

3 استادیار گروه علــوم خاک، دانشکده کشاورزی دانشگاه ارومیه

چکیده

فسفر یکی از مهم­ترین عناصر محدودکننده رشد گیاهان می­باشد. استفاده از پتانسیل ریزجانداران حل‌کننده‌ فسفات­های نامحلول یکی از راهکارهای مهم در تأمین نیاز فسفری گیاهان است. غربال‌گری توانایی انحلال ریزجانداران در شرایط آزمایشگاهی به­منظور استفاده در کودهای زیستی، تحت تأثیر ترکیب عناصر غذایی محیط می‌باشد.  هدف از این مطالعه مدل­سازی تأثیر منابع و سطوح مختلف کربن و نیتروژن و میزان تری­کلسیم فسفات بر انحلال فسفر با استفاده از طرح پلاکت – برمن و روش سطح پاسخ بر مبنای طرح مرکب مرکزی بود. ابتدا به­منظور غربالگری و شناسایی منابع تأثیرگذار کربن و نیتروژن بر انحلال فسفر توسط باکتری Pseudomonas fluorescens، تعداد 12 آزمایش بر اساس طرح پلاکت – برمن اجرا شد. نتایج نشان داد که از بین منابع نیتروژن، عصاره مخمر تأثیر بیشتری در انحلال فسفر داشت. براساس نتایج این بخش، اثر سطوح متفاوت ساکارز، عصاره مخمر و همچنین تری­کلسیم فسفات بر انحلال فسفر با استفاده از طرح مرکب مدل­سازی شد.  نتایج تجزیه واریانس بیان­گر کارآمدی بالای (g L-1­0372/0= RMSE و 896/0= R2) مدل طرح مرکب مرکزی در برآورد انحلال فسفر مشاهده­ای بود. عصاره مخمر و تری­کلسیم­فسفات به­ترتیب مؤثرترین عوامل تأثیرگذار بر انحلال فسفر بودند. به­طوری­که افزایش مقدار عصاره مخمر در محدوده­ صفر تا 4 گرم بر لیتر و تری­کلسیم­فسفات در محدوده­ صفر تا 20 گرم بر لیتر سبب افزایش غلظت فسفر محلول شد. براساس مدل طرح مرکب مرکزی غلظت­های 58/18، 4 و 97/17 گرم بر لیتر از ساکارز، عصاره مخمر و تری­کلسیم فسفات به­عنوان شرایط بهینه برای دست­یابی به بیشینه غلظت فسفر محلول در محیط کشت پیش­بینی شد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Modeling Phosphate Solubilization by Pseudomonas fluorescens Using Response Surface Methodology

نویسندگان [English]

  • S Ashrafi-Saeidlou 1
  • MH Rasouli-sadaghiani 2
  • F Asadzadeh 3
  • M Barin 3
چکیده [English]

Phosphorus is one of the most important limiting elements for plant growth. Using phosphate solubilizing microorganisms (PSM) is one of the main strategies to meet plant P demand. In Vitro screening of phosphate solubilization potential in order to use as biofertilizers, is influenced by nutrient composition of media. The purpose of this study was modeling the effects of different carbon and nitrogen sources and tricalcium phosphate on phosphorous solubilization using Placket-Burman design and response surface methodology with a central composite design. At the first step, 12 experiments based on Placket-Burman design were carried out to screen and identify the effective carbon and nitrogen sources in phosphorous dissolution by Pseudomonas fluorescens. Results indicated that yeast extract was more effective source of nitrogen in comparison with other nitrogen sources. According to the results of the first step, response surface methodology with central composite design was employed to evaluate and to model the effects of sucrose, yeast extract and tricalcium phosphate concentrations on phosphate dissolution. The analysis of variance (ANOVA) depicted the high performance of the central composite predictive model of phosphorus dissolution (R2= 0.896 and RMSE=0.0372 g L-1). The yeast extract and tricalcium phosphate were the most significant parameters for phosphate solubilization. Increasing of the yeast extract concentration at the range of 0-4 g L-1 and the tricalcium phosphate at the range of 0-20 g L1 significantly increased the soluble phosphate concentration. According to central composite design, maximum phosphate dissolution was obtained at the yeast extract, sucrose and tricalcium phosphate concentrations of 4, 18.58, 17.97 g L1 respectively.
 

کلیدواژه‌ها [English]

  • Central composite design
  • Media
  • Modeling
  • Phosphate Solubilizing Bacteria
  • Placket-Burman

مراجع

Abd-Alla MH, 1994. Phosphate and the utilization of organic phosphorous by Rhizobium Leguminosarum biovar viceae. Lett Appl Microbiol 18: 294-296.
Aghaeinejad-Meybodi A, EbadiA, Shafiei S, Khataee and Rostampour M, 2015. Degradation of antidepressant drug fluoxetine in aqueous media by ozone/H2O2 system: process optimization using central composite design. Environ Technol 36(12):1477-1488.
Amanpour J, Salari D, Niaei A, Mousavi SM and Panahi PN, 2013. Optimization of Cu/activated carbon catalyst in low temperature selective catalytic reduction of NO process using response surface methodology. J Environ Sci Health., Part A 48(8): 879-886.
Arulanantham R, Pathmanathan S, Ravimannan N and Niranjan K, 2012. Alternative culture media for bacterial growth using different formulation of protein sources. J Nat Prod Plant Resour 2(6): 697-700.‏
Banik S and Dey BK, 1982. Available phosphate content of an alluvial soil as influenced by inoculation of some isolated phosphate-solubilizing microorganisms. Plant Soil 69: 353-364.
Cotteni A, 1980. Methods of plant analysis. Pp. 64-100. In: Robert Lee Westerman. Soil and Plant Testing, FAO Soil Bulletin.
Cunningham JE and Kuiack C, 1992. Production of citric acid and oxalic acid and solubilization calcium phosphate by penicillium billai. Appl Environ Microbiol 58: 1451–1458.
Fasim F, Ahmed N, Parson R and Gadd GM, 2002. Solubilization of zinc salts by a bacterium isolated from air environment of a tannery. FEMS Microbiol Lett 213: 1–6.
Goldstein AH, 1986. Bacterial solubilization of mineral phosphates: historical perspective and future prospects. Am J Altern Agri 1: 51-57.
Gangoliya SS, Kishor G and Singh ND, 2015. Phytase production through response surface methodology and molecular characterization of Aspergillus fumigatus NF191. Indian J Exp Biol 53: 350-355.
Illmer P and Schinner F, 1992. Solubilization of hardly-soluble AlPO4 with P-solubilizing microorganisms. Soil Bio and Biochem 24: 389-395.
Illmer P, Barbato A and Schinner F, 1995. Solubilization of hardly soluble AlPO4 with P Solubilizing Microorganism. Soil Biol Biochem 27: 265-270.
Isaie M and Padmavathi T, 2015. Statistical optimization of cellulase and xylanase enzyme production by Penicillium crustosum using sugar beet peel substrate by response surface methodology. Res J Pharm BiolChem Sci 6: 1144-1151.
Kucey RMN, 1983. Phosphate – solubilizing bacteria and fungi in various cultivated and virgin Alberta soils. Can J Soil Sci 63: 671- 678.
Mousavi SM, Niaei A, Salari D, Nakhostin-Panahi P and Samandari M, 2013. Modelling and optimization of Mn/activate carbon nanocatalysts for NO reduction: comparison of RSM and ANN techniques. Environ Techno 34(11): 1377-84.
Myers RH and Montgomery DC, 2002. Response Surface Methodology: Process and Product Optimization using Designed Experiments, (2end ed.), John Wiley and Sons, UK.
Nguyen C, Yan W, Le Tacon F and Lapyire F, 1992. Genetic variability of phosphate solubilizing activity by monocaryotiv and dicaryotic mycelia of the ectomycorrhizal fungus laccaria bicolor (Maire) P D Orton. Plant Soil 143:193-199.
Padmavathi T, 2015. Optimization of phosphate solubilization by Aspergillus niger using plackett-burman and response surface methodology. Soil Sci Plant Nutr 15 (3): 781-793.
Plackett RL and Burman JP, 1946. The Design of optimum multifactorial experiments. Biometrika 33:305-325.
Pikovskaya RI, 1948. Mobilization of phosphorous in soil connection with the vital activity of some microbial species. Microbiol 17: 362-370.
Pradhan Nand Sukla L.B, 2005. Solubilization of inorganic phosphate by fungi isolated from agriculture soil. Afr J Biotechnol 5: 850-854.
Prasad MP, 2014. Optimization of fermentation conditions of phosphate solubilizing bacteria- a potential bio fertilizer. MRJMBS 2(2): 031-035.
Rajendran A, Meikandhan T and Viruthagiri T, 2007. Statistical evaluation of medium components by Plackett- Burman experimental design and kinetic modeling of lipase production by Pseudomonas fluorescens. Indian J Biotechnol 6: 469- 478.
Rodriguez H, and Fraga R, 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol Adv 17: 319-339.
Sagervanshi A, Kumari P, Nagee A and Kumar A, 2012. Media optimization for inorganic phosphate solubilizing bacteria isolated from Anand agriculture soil. Int J pharm Life sci 2: 2250-0480.
Sangeeta M, and Nautiyal CS, 2001. An efficient method for qualitative screening of phosphate-solubilizing bacteria. Curr. Microbiol 43:51-56.
Seilsepour M, Baniani E and Kianirad M, 2002. Effect of Phosphate Solubilizing Microorganism (PSM) in reducing the rate of phosphate fertilizers application to cotton crop. Proceedings of the 15th International Meeting on Microbial Phosphate Solubilization Salamanca University, 16-19 July, Salamanca, Spain.
Shahab S and Ahmed N, 2008. Effect of various parameters on the efficiency of zinc phosphate solubilization by indigenous bacterial isolates. Afr J Biotechnol 7 (10): 1543-1549.
Sharmila M, Ramanand K and Sethunathan N, 1989. Effect of yeast extract on the degradation of organophosphorus insecticides by soil enrichment and bacterial cultures. Can J Microbiol 35: 1105-1110.
Sheydaei M, Aber S and Khataee AR, 2014. Degradation of amoxicillin in aqueous solution using nanolepidocrocite chips/H2O2/UV: optimization and kinetics studies. Ind Eng Chem Res 20:1772–1778.
Swetha S, VarmaA and Padmavathi T, 2014. Statistical evaluation of the medium components for the production of high biomass, a-amylase and protease enzymes by Piriformospora indica using Plackett–Burman experimental design. Biotech 4: 439–445.
Thakur D, Kaur M and Shyam V, 2014. Optimization of best cultural conditions for high production of phosphate solubilizing activity by Fluorescent Pseudomonas isolated from normal and replant sites of apple and pear. Bioscan 9: 143-150.
Todar K, 2004. Todars Online Textbook of Bacteriology. University of Wisconsin Madison. Department of Bacteriology, Wisconsin, USA.
Whitelaw MA, Harden TJ and Helyar KR, 1999. Phosphate solubilization in solution culture by the soil fungus Penicillium radicum. Soil Biol Biochem 31: 655- 665.
Whitelaw MA, 2007. Growth promotion of plants inoculated with phosphate solubilizing fungi. Adv Agronomy 69: 99-151.
Xia J, Knight D and Leggett M, 2009. Comparison of media used to evaluate Rhizobium leguminosarum bivar viciae for phosphate solubilizing ability. Can J Microbiol 55: 910–915.

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