مدل‌سازی انحلال فسفر توسط Pseudomonas fluorescens با استفاده از روش سطح پاسخ

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری گروه علــوم خاک، دانشکده کشاورزی دانشگاه ارومیه

2 دانشیار گروه علــوم خاک، دانشکده کشاورزی دانشگاه ارومیه

3 استادیار گروه علــوم خاک، دانشکده کشاورزی دانشگاه ارومیه

چکیده

فسفر یکی از مهم­ترین عناصر محدودکننده رشد گیاهان می­باشد. استفاده از پتانسیل ریزجانداران حل‌کننده‌ فسفات­های نامحلول یکی از راهکارهای مهم در تأمین نیاز فسفری گیاهان است. غربال‌گری توانایی انحلال ریزجانداران در شرایط آزمایشگاهی به­منظور استفاده در کودهای زیستی، تحت تأثیر ترکیب عناصر غذایی محیط می‌باشد.  هدف از این مطالعه مدل­سازی تأثیر منابع و سطوح مختلف کربن و نیتروژن و میزان تری­کلسیم فسفات بر انحلال فسفر با استفاده از طرح پلاکت – برمن و روش سطح پاسخ بر مبنای طرح مرکب مرکزی بود. ابتدا به­منظور غربالگری و شناسایی منابع تأثیرگذار کربن و نیتروژن بر انحلال فسفر توسط باکتری Pseudomonas fluorescens، تعداد 12 آزمایش بر اساس طرح پلاکت – برمن اجرا شد. نتایج نشان داد که از بین منابع نیتروژن، عصاره مخمر تأثیر بیشتری در انحلال فسفر داشت. براساس نتایج این بخش، اثر سطوح متفاوت ساکارز، عصاره مخمر و همچنین تری­کلسیم فسفات بر انحلال فسفر با استفاده از طرح مرکب مدل­سازی شد.  نتایج تجزیه واریانس بیان­گر کارآمدی بالای (g L-1­0372/0= RMSE و 896/0= R2) مدل طرح مرکب مرکزی در برآورد انحلال فسفر مشاهده­ای بود. عصاره مخمر و تری­کلسیم­فسفات به­ترتیب مؤثرترین عوامل تأثیرگذار بر انحلال فسفر بودند. به­طوری­که افزایش مقدار عصاره مخمر در محدوده­ صفر تا 4 گرم بر لیتر و تری­کلسیم­فسفات در محدوده­ صفر تا 20 گرم بر لیتر سبب افزایش غلظت فسفر محلول شد. براساس مدل طرح مرکب مرکزی غلظت­های 58/18، 4 و 97/17 گرم بر لیتر از ساکارز، عصاره مخمر و تری­کلسیم فسفات به­عنوان شرایط بهینه برای دست­یابی به بیشینه غلظت فسفر محلول در محیط کشت پیش­بینی شد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Modeling Phosphate Solubilization by Pseudomonas fluorescens Using Response Surface Methodology

نویسندگان [English]

  • S Ashrafi-Saeidlou 1
  • MH Rasouli-sadaghiani 2
  • F Asadzadeh 3
  • M Barin 3
چکیده [English]

Phosphorus is one of the most important limiting elements for plant growth. Using phosphate solubilizing microorganisms (PSM) is one of the main strategies to meet plant P demand. In Vitro screening of phosphate solubilization potential in order to use as biofertilizers, is influenced by nutrient composition of media. The purpose of this study was modeling the effects of different carbon and nitrogen sources and tricalcium phosphate on phosphorous solubilization using Placket-Burman design and response surface methodology with a central composite design. At the first step, 12 experiments based on Placket-Burman design were carried out to screen and identify the effective carbon and nitrogen sources in phosphorous dissolution by Pseudomonas fluorescens. Results indicated that yeast extract was more effective source of nitrogen in comparison with other nitrogen sources. According to the results of the first step, response surface methodology with central composite design was employed to evaluate and to model the effects of sucrose, yeast extract and tricalcium phosphate concentrations on phosphate dissolution. The analysis of variance (ANOVA) depicted the high performance of the central composite predictive model of phosphorus dissolution (R2= 0.896 and RMSE=0.0372 g L-1). The yeast extract and tricalcium phosphate were the most significant parameters for phosphate solubilization. Increasing of the yeast extract concentration at the range of 0-4 g L-1 and the tricalcium phosphate at the range of 0-20 g L1 significantly increased the soluble phosphate concentration. According to central composite design, maximum phosphate dissolution was obtained at the yeast extract, sucrose and tricalcium phosphate concentrations of 4, 18.58, 17.97 g L1 respectively.
 

کلیدواژه‌ها [English]

  • Central composite design
  • Media
  • Modeling
  • Phosphate Solubilizing Bacteria
  • Placket-Burman
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