مقایسه اثر سویه وحشی سودوموناس مندوسینا و سویه تراریخت با قابلیت تولید ACCدآمیناز بالا بر تحریک جوانه زنی بذر و رشد گیاهان گوجه فرنگی و گندم

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار گروه زیست شناسی، دانشگاه پیام نور

2 پروفسور گروه ژنتیک، دانشکده زیست شناسی، دانشگاه دولتی بلاروس، مینسک، بلاروس

چکیده

آنزیم آمینوسیکلوپروپان کربوکسیلات دآمیناز با کاهش تولید اتیلن در گیاه، سبب افزایش رشد می‌گردد. دراین تحقیق، اثر یک سویه تراریخت سودوموناس مندوسینا با توانایی افزایش یافته تولید این آنزیم، بر جوانه‌زنی بذر دو گیاه تک‌لپه و دو‌‌لپه در پتری و نیز تحریک رشد در گلدان بررسی شد. ظروف پتری حاوی بذور گیاهان گوجه‌فرنگی و گندم  به 3 گروه 5 تایی تقسیم شدند.عمق آبیاری کاربردی روزانه 10 میلی لیتر برای هر ظرف و شامل سه تیمار آبیاری با آب مقطر، آب حاوی باکتری وحشی و آب حاوی باکتری تراریخت بوده و به‌تدریج طی 5 روز صورت گرفت. جهت ارزیابی اثر باکتری روی تحریک رشد گندم و گوجه‌فرنگی، جوانه‌های هم‌اندازه انتخاب و در سه گروه گلدان شامل شاهد، تیمار با باکتری وحشی و تراریخت کاشته شدند. پس از گذشت 5 هفته از رشد، سه شاخص طول ساقه، طول ریشه و بیومس بررسی گردیدند. در تیمار بذر، طول ساقه جوانه گوجه‌فرنگی تلقیح شده با باکتری تراریخت (تست)  2±50% بیش از طول ساقه شاهد و 3±2/31% بیش از نمونه تلقیح شده با باکتری وحشی بود. طول ریشه نمونه تست1±6/28% بیش از شاهد و بیومس تست3±1/34% بیش از شاهد بود (p <0.05). در تلقیح جوانه، طول ساقه گوجه‌فرنگی تست در گلدان2±50% بیش از طول ساقه شاهد و1±2/31% بیش از نمونه تلقیح شده با باکتری وحشی بود. طول ریشه تست،4±6/28% بیش از شاهد و بیومس تست نیز3±1/34% بیش ازشاهد بود(05/0p <). باکتری سودوموناس مندوسینا تراریخت به‌صورت معنی‌داری سبب تحریک و افزایش رشد بذر و گیاه از نظر طول ساقه، طول ریشه و بیومس گردید. تمام شاخص‌ها اندازه گیری شده در بذر و گیاه گوجه‌فرنگی بیش از گندم بود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

A Comparative Study on the Effects of Wild form and Transformant Pseudomonas mendocina With Enhanced ACC Deaminase Production on Seed Germination and Growth of Tomato and Wheat Plants

نویسندگان [English]

  • M sadrnia 1
  • N Maksimava 2
چکیده [English]

Amino cyclopropane carboxylate (ACC) deaminase enzyme can increase plant growth by reducing the ethylene production in plants. In this study the effect of genetically modified strain of Pseudomonas mendocina with enhanced enzyme production property was evaluated on the seed germination of monocots and dicot plants in petries and also on the stimulation of plant growth in pots. Petries containing tomato and wheat seeds were divided into 3 groups of 5. All petries were irrigated with 10 ml of any distilled water, water containing wild and transgenic strains for 5 days, gradually. In stimulating growth study by the bacterium on the growth of tomato and wheat plants, the same-sized plants were selected and divided into three pots groups consisting of control, wild and transgenic. After 5 weeks of growth, stem length, root length and biomass content were assessed. Results of the tests showed that, stem lengths of tomato seedlings inoculated with genetically modified bacteria (test) were 50±2% more than stem lengths of control and 31.2±3% more than the samples inoculated with wild bacteria. Root lengths of the test samples were 28.6±1% more than those of the control and biomass quantities of the test were 34.1±3% more than those of the control (p < 0.05). Results of the experiments revealed that shoot lengths of test tomatoes in pots were 50±2% more than stem lengths of the control and were 31.2±1% more than stem lengths of the sample inoculated by wild bacteria. The root lengths of the test tomatoes were 28.6±4% more than those of the control and biomass quantities of the test tomatoes were 34.1±3% more than those of the control (p < 0.05). The results of experiments on seeds and wheat plant inoculated by genetically modified P. mendocina revealed that the bacterium significantly promoted the growth and biomass in comparison with the wild samples. All the properties measured on tomato seeds and plants were higher than those of wheat.

کلیدواژه‌ها [English]

  • Amino cyclopropane carboxylate deaminase
  • Pseudomonas mendocina
  • Transformant
  • Tomato
  • Wheat

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