اثر قارچ‌های میکوریز آربوسکولار بر گیاه‌پالایی سرب توسط سورگوم (Sorghum bicolor L.)

نوع مقاله: مقاله پژوهشی

نویسندگان

دانشکده کشاورزی دانشگاه تبریز

چکیده

          همزیستی گیاه با قارچ­های میکوریز آربوسکولار در خاک‌های آلوده به فلزات سنگین می­تواند رشد، تغذیه و مقاومت گیاه به فلزات سنگین را تحت تأثیر قرار داده و نقش مهمی در گیاه­پالایی داشته باشد. در این تحقیق تأثیر همزیستی با دو گونۀ قارچ گلوموس اینترارادیسز و گلوموس موسه بر جذب و انتقال سرب در گیاه سورگوم در شرایط گلخانه­ای بررسی گردید. آزمایش به صورت فاکتوریل و در قالب طرح طرح پایۀ کاملاً تصادفی با چهار سطح سرب (صفر، 200، 400 و 600 میلی­گرم سرب بر کیلوگرم خاک) و سه تیمار قارچ (دو گونۀ میکوریز و شاهد غیرمیکوریز) با چهار تکرار انجام شد. برای ایجاد تیمارهای سرب از نمک نیترات سرب استفاده شد. تلقیح گیاهان با هر دو گونۀ قارچی­ انتقال سرب به بخش هوایی را به­طور معنی­داری کاهش داد. همزیستی با قارچ گلوموس اینترارادیسز سبب افزایش وزن خشک بخش هوایی در سطح 200 میلی­گرم سرب بر کیلوگرم خاک و افزایش وزن خشک ریشه در سطوح 400 و 600 میلی­گرم سرب بر کیلوگرم خاک در مقایسه با گیاهان غیرمیکوریزی گردید ولی اثر معنی­داری بر سایر پارامتر­های رشد نداشت. در سطوح بالای سرب، همزیستی با قارچ گلوموس موسه سبب کاهش معنی­دار (p<0.05) وزن تر و خشک گیاه، سطح برگ و شاخص کلروفیل برگ­ها در مقایسه با گیاهان غیرمیکوریزی گردید. در سطوح 400 و 600 میلی­گرم سرب بر کیلوگرم خاک، نسبت غلظت سرب ریشه به بخش هوایی گیاهان در همزیستی با قارچ گلوموس موسه به­ طور
معنی­داری بیشتر از گیاهان بدون میکوریزی و گیاهان تلقیح شده با گلوموس اپنترارادیسز بود. 

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Arbuscular Mycorrhizal Fungi on Lead Phytoremediation by Sorghum (Sorghum bicolor L.)

نویسندگان [English]

  • S Amanifar
  • N Aliasgharzad
  • N Najafi
  • Sh Oustan
  • S Bolandnazar
چکیده [English]

          Arbuscular mycorrhizal fungi in association with plant roots may affect plant growth, nutrition and tolerance to heavy metals in polluted soils. Therefore, this association can play an important role in phytoremediation. In this study,theeffects of two arbuscular mycorrhizal fungi species including Glomus intraradices and Glomus mosseae, on the uptake and translocation of Pb in sorghum, were investigated under greenhouse conditions. The experiment was conducted as factorial, completely randomized design including four levels of Pb2+ (0, 200, 400 and 600 mg Pb2+ kg-1) and three treatments of fungi (two mycorrhizal fungi species and a non-mycorrhizal plant) with four replications. Lead were added to the pots as Pb(NO3)2.The Results indicated that symbiosis with G. intraradices increased shoot dry weight at 200 and root dry weight at 400 and 600 mg Pb kg-1 compared to the non-mycorrhizal treatment. There were, however, no significant effects on other growth parameters. Both mycorrhizal fungi caused a significant decrease in Pb2+ translocation from the root to the shoot. However, the shoot and root dry weights, leaf area and chlorophyll content in plants inoculated with G. mosseae at higher levels of Pb2+ were considerably (p<0.05) decreased compared to the non-mycorrhizal plants. At pb levels of 400 and 600 mgkg-1, the ratio of Pb concentration in root to that of shoot in plants inoculated with G. mosseae was significantly (p<0.05) greater than that of plants inoculated with G. intraradices and none inoculated ones.

کلیدواژه‌ها [English]

  • Arbuscular mycorrhizae
  • Heavy metal
  • Lead
  • Phytoremediation
  • Sorghum
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