Assessment of Biodegradation of Gas Oil, Toluene and Phenantherene in Presence of Pseudomonas Fluorescens CHAO, Pseudomonas Putida P13 and P5 Pantoea Agglomerans

Document Type : Research Paper

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Abstract

  Soil and water contamination by oil is one of the great environmental concerns. Bioremediation is one of principal strategies for remediation, wherein the pollutants can be removed by use of microorganism or any biological process that uses native or exogenous microorganisms. According to the positive role of Pseudomonas genus in bioremediation, in this study the potential of two Pseudomonad bacteria, including Peudomonas fluorescens CHAO, P. putida P13 and a non-Pseudomonad Pantoea agglomerans as hydrocarbon utilizing bacteria were estimated in presence of three compounds including gas oil (2%), toluene (1%) and phenanthrene (0.05%). Bacterial inoculum containing 108 cfu/ml was used in plate and liquid assays, which were performed in factorial experiment based on completely randomized design with three replications. Results showed that in CFMM plate assay, diameter of colony is not affected by any of bacterial isolates and compounds. In broth assay,  P. putida P13 and P. fluorescence CHAO had similar behavior in the presence of Gas oil, toluene and phenanthrene and trend of bacterial growth was the same at different times, while with P. agglomerans P5 the lowest number of bacteria was achieved. Among three compounds the highest degradability was recorded to the gas oil, followed by phenanthrene and toluene. The ability of bacteria in degradation of oil-compounds increased by increasing time of incubation. To conclude, this study suggests the potential use of two Pseudomonas isolates for bioremediation of hydrocarbon-contaminated environments especially with aliphatic hydrocarbon.

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