Effect of Humic Acid Application Method on Bioavailability of Iron in Canola

Authors

1 M.Sc. Graduate, Dept. of Soil Science, Gorgan Uni. of Agri. Sci.and Natural Res. Iran.

2 Assoc. Prof, Dept. of Soil Science, Gorgan Uni. of Agri. Sci.and Natural Res. Iran.

3 Assist. Prof, Dept. of Soil Science, Gorgan Uni. of Agri. Sci.and Natural Res. Iran.

4 Phd. Students, Dept. of Soil Science, Gorgan Uni. of Agri. Sci.and Natural Res. Iran.

Abstract

In order to investigate the effect of acid humic application method including soil application, spraying and fertigation on iron (Fe) supplying in rapeseed, an expriment was caried out consisting of 10 treatments and 4 replications. Treatments included humic acid soil application in three levels (1, 2 and 4 g kg-1 soil), spraying at three levels (0.1, 0.2 and 0.4%) and with fertigation at three levels (1000, 2000 and 4000 mg L-1) and control treatment (without humic acid). The results showed that the highest total iron and active iron concentration in stalk were 85 and 44.9 mg kg-1, respectively, in the treatment of 2000 mg L-1 of humic acid with fertigation, and the lowest value, respectively, with an average of 54.62 and 20.40 mg kg-1 for control treatment. Also, the order of significance for extractable- Fe from the pot soil by various extractants was as follows: rapid ammonium oxalate > hydroxylamine hydrochloride>AC-EDTA> DTPA> AB-DTPA >EDTA. The results obtained in the present study showed that extracted iron was significantly correlated with chlorophyll content by most of the extractants. Among extractants, only extracted Fe with DTPA correlated positively and significantly with total Fe (r=0.69*) and active Fe (r=0.79*) while, total iron of leaf had weak and non-signifcant correlation (r= 0.35) with DTPA. Based on the results of this study, active iron measurement can be a suitable method for predicting iron deficiency in canola plants.

Keywords

References

Abadia J, Millan E., Montanes L and Heras L, 1980. DTPA and NH4HCO3-DTPA extractable Fe, Mn and Zn levels in the Ebro Valley. Anales de la Estacion Experimental de Aula Dei 15(1): 181-193.
Abou-Aly HE and Mady MA, 2009. Complemented effect of humic acid and biofertilizers on wheat (Triticum aestivum L.) productivity. Annals of Agricultur Science Moshtohor 47(1): 1-12.
Adiloglu A, 2006. Determination of suitable chemical extraction methods for the available iron content of brown forest soils in Turkey. Eurasian Soil Science 39(9): 961-967.
Aiken GR, McKnight DM, Wershaw RL and MacCarthy P, 1985. Humic substances in soil, sediment, and water: geochemistry, isolation, and characterization. Wiley-Interscience publication.
Al-Mustafa W, Abdallah A and Falatah A, 2001. Assessment of five extractants for their ability to predict iron uptake and response of sorghum grown in calcareous soils. Communications in Soil Science and Plant Analysis 32(5-6): 907-919.
Ammari TG and Mengel K, 2006. Total soluble Fe in soil solutions of chemically different soils. Geoderma 136: 876–885.
Antonio SS, Jua SA, Margarita J, Juana J and Dolores B, 2006. Improvement of iron uptake in table Grapeby addition of humic substances. Journal of Plant Nutrition 30(1): 1-7.
Astaraei AR and Ivani R, 2008. Effect of organic sources as foliar spray and root madia on nutrition in cowpea plant. American-Eurasian Journal of Agricultural and Environmental Sciences 3: 352-356.
Baissa T, Suwanarit A, Ososapar Y and Sarobol E, 2007. Status of Mn, Fe, Cu, Zn, B and Mo in Rift valley soils of Ethiopia: Laboratory assessment. Kasetsart Journal (Natural Science) 41(1): 84-95.
Bar-Akiva A, 1986. Induced formation of enzymes as a possible measure of micronutrient requirement of citrus trees. Control de la Fertilizacion de lad Plantas Cultivadas; CEBAC: Sevilla, Spain 573–581.
Barani Motlagh M and Towfighi H, 2010. Comparison of kinetic equations to describe the release of iron from some calcareous soils. Iranian Journal of Soil and Water Research 1(40): 43-50. (In Persian with English Abbstract)
Benítez M, Pedrajas V, Del Campillo M and Torrent J, 2002. Iron chlorosis in olive in relation to soil properties. Nutrient Cycling in Agroecosystems 62(1): 47-52.
Berg Marlene G and Hugh Gardner E, 1978. Methods of soil analysis used in the soil testing laboratory at Oregon State University. Corvallis, Or. Agricultural Experiment Station, Oregon State University, 89:4.16.
Bremner JM and Mulvaney CS, 1982. Nitrogen—total. Pp. 595-624. In: Page AL, Miller RH and Keeney DR (Eds). Methods of soil analysis. Part 2. Chemical and microbiological properties, American Society of Agronomy, Soil Science Society of America, Madison, WI.
Brown AL, Quick J and Eddings JL, 1971. A comparison of analytical methods for soil zinc. Soil Science Society America Proceedings 35: 105- 107.  
Chapman HD, 1965. Cation exchange capacity. Pp891- 901. In: Black CA (ed), Methods of Soil Analysis. Part 2. American Society of Agronomy. Madison, WI.
Chen Y and Barak P, 1982. Iron nutrition of plants in calcareous soils. Advance in Agronomy 35 (628): 217-240.
De Santiago A and Delgado A, 2006. Predicting iron chlorosis of lupin in calcareous Spanish soils from iron extracts. Soil Science Society of America Journal 70(6): 1945-1950.
De Santiago A and Delgado A, 2007. Effects of humic substances on iron nutrition of lupin. Biology and Fertility of Soils 43:829–836.
De Santiago A, Díaz I, Del Campillo MC, Torrent J and Delgado A, 2008. Predicting the incidence of iron deficiency chlorosis from hydroxylamine-extractable iron in soil. Soil Science Society of America Journal 72(5): 1493-1499.
Del Campillo MC and Torrent J, 1992. A rapid acid‐oxalate extraction procedure for the determination of active Fe‐oxide forms in calcareous soils. Zeitschrift für Pflanzenernährung und Bodenkunde 155(5): 437-440.
Delfine S, Tognetti R, Desiderio E and Alvino A, 2005. Effect of foliar application of N and humic acids on growth and yield of durum wheat. Agronomy for Sustainable Development 25: 183-191.
Derek R and Phillips JP, 1987. Rapid assay for microbially reducible ferric iron in aquatic sediments. Applied and Environmental Microbiology 53 (7): 1536-1540.
Drouineau G, 1942. Dosage rapide du calcaire actife du sol; Nouvelles donnees sur la separation et la nature des fractions calcaires. Annuals of Agronomy 12: 441-450.
Dursun A, Guvenc I and Turan M, 2002. Effect of different levels of humic acid on seedling growth and macro and micronutrient contents of tomato and eggplant. Acta Agrobotanical 56: 81-88.
Ebrahimi S, Bahrami HA, Homaei M and Malakoti MJ, 2005. The role of organic matter in increasing the soil fertility level. High Council for Policy Development of Application of Biological Materials and Optimal Use of Fertilizer and Poison in Agriculture. Technical Journal No. 401. (In Persian with English Abbstract)
El-Bassiony AM, Fawzy ZF, Abd El-Baky MMH and Mahmoud Asmaa R, 2010. Response of snap bean plants to mineral fertilizers and humic acid application. Research Agricultural and Biological Science 6(2): 169-175.
Emami M and Dordipour E, 2012. Selection of suitable extractant to extract available iron in peach in soils of Golestan Province. Journal of Soil Managmenet and Sustainable Production 2(2): 89-103. (In Persian with English Abbstract)
Fageria NK, 2010. The use of nutrients in crop plants, CRC Press.
Foy CD, Fleming AL and Schwortz JW, 1981. Differential resistance of weeping love grass genotypes to iron related chlorosis. Journal of Plant Nutrition 3: 537-550.
Gee GH and Bauder JW, 1986. Particle size analysis, Pp. 383-409. In: Klute A, (Ed), Methods of Soil Analysis. Part 2, Physical properties. Soil Science Society of America, Madison, WI.
Hakan C, Vahap Katkat A, Bulent Asık B and Turan MA, 2011. Effect of foliar applied humic acid to dry weight and mineral nutrient uptake of maize under calcareous soil conditions. Communications in Soil Science and Plant Analysis 42(1): 29 – 38.
Hammer D and Keller C, 2002. Changes in the rhizosphere of metal accumulating plants evidenced by chemical extractants. Journal of Environmental Quality 31: 1561-1569.
Havlin J, Beaton JD, Tisdale SL and Nelson WL, 1999. Soil Fertility and Fertilizers, 6 Ed. Soil Science Society of America. Madison, WI.
Jackosn ML, 1973. Soil Chemical Analysis. Prentice Halla of India Private Limited. New Delhi, India.
Jahan M, Ghale Nui Sh, Khamoshi A and Amiri MB, 2015. Investigation of basal eagroacological features (Ocimum basilicum L.) under the influence of superabsorbent application of moisture, ecosystems and irrigation periods. Journal of Horticulture 29(2): 240-254.
James DW, 1984. General summary of the second international symposium on iron nutrition and interaction in plants. Journal of Plant Nutrition 7: 859-864.
Jansen B, Tonneijck FH and Verstraten JM, 2011. Selective extraction methods for aluminium, iron and organic carbon from Montane volcanic ash soils. Pedosphere 21(5): 549–565.
Jones Jr JB and Case VW, 1990. Sampling, handling and analyzing plant tissue samples. Pp. 389-427. In: Westerman RL, (Ed), Soil testing and plant analysis. Soil Science Society of America, Madison, WI.  
Katyal JC and Sharma BD, 1980. A new technique of plant analysis to resolve iron chlorosis. Plant and Soil 55(1): 105-119.
 Khalkhal K, Reyhanitabar A and  Najafi N, 2015. Evaluation of extraction methods for determination of iron usable use of maize in calcareous soils of East Azarbaijan Province. Iranian Journal of Water and Soil Research 47(2): 427-437. (In Persian with English Abbstract)
Khoram Ghahfarokhi A, Rahimi A, Torabi B and Maddah Hosseini Sh. 2015. Effect of humic acid application and foliar spraying of compost tea and vermiwash on nutrient absorption and chlorophyll content of safflower (Carthamus tinctorius L.). Journal of Oil Plants Production 2 (1): 71-84. (In Persian with English Abbstract)
Lindsay WL, 1984. Soil and plant relationships associated with iron deficiency with emphasis on nutrient interactions. Journal of Plant Nutrition 7(1-5): 489-500.
Lindsay WL, 1991. Iron oxide solubilization by organic matter and its effect on iron availability. Pp. 29-36. In: Chen Y and Hadar Y (Eds). Iron Nutrition and Interactions in Plants. Kluwer Academic Publishers.
Lindsay WL and Norvell WA, 1978. Development of a DTPA soil test for zinc, iron, manganese, and copper. Soil Science Society of America Journal 42(3): 421-428.
Maccarthy P, 2001. The principles of humic substances. Soil Science 166(11): 738-751.
Mahashabde JP and Patel S, 2012. DTPA-Extractable micronutrients and fertilitystatus of soil in shirpur Tahasil region. International Journal of ChemTech Research 4(4): 1681-1685.
Malakouti MJ, Keshavarz P and Karimian NA, 2008. A comprehensive approach towards identification nutrients deficiencies and optimal fertilization for sustainable agriculture. Tarbiat Modares University Publication. (In Persian with English Abbstract)
Marschner H and Marschner P, 2012. Mineral nutrition of higher plants, Academic press.
Matocha JE and Pennington D, 1982. Effect of plant iron recycling on iron chlorosis of grain sorghum growth on calcareous soils. Journal of Plant Nutrition 5: 869-882.
Miller GW, Pushnik JC and Welkie GW, 1984. Iron chlorosis, a worldwide problem, the relation of chlorophyll biosynthesis to iron. Journal of Plant Nutrition 7: 1-22.
Nardi S, Pizzeghello D, Muscolo A and Vianello A, 2002. Physiological effects of humic substances on higherplants. Soil Biology and Biochemistry 34 (11): 1527-1536.
Nasooti miandoab R, Samavat S and Tehrani MM, 2010. Humic acid fertilizer on plants and soil properties. Agric. Food. 101: 53-55. (In Persian with English Abbstract)
Neaman A and Aguirre L, 2007. Comparison of different methods for diagnosis of iron deficiency in avocado. Journal of Plant Nutrition 30: 1098 – 1108.
Olsen SR 1954. Estimation of available phosphorus in soils by extraction with sodium bicarbonate. United States Department of Agriculture; Washington.
Page AL, Miller RH and Keeney DR, 1982. Chemical and microbiological properties. Methods of Soil Analysis. Part 2. American Society of Agronomy, Soil Science Society of America, Madison, WI.
Parfitt R and Childs C, 1988. Estimation of forms of Fe and Al-a review, and analysis of contrasting soils by dissolution and Mossbauer methods. Soil Research 26(1): 121-144.
Paya-Perez A, Sala J and Mousty F, 1993. Comparison of ICPAES and ICP-MS for the analysis of trace elements in soil extracts. International Journal of Environmental Analytical Chemistry 51: 223-230.
Rafati F, 2004. The effect of different additives on iron availability in calcareous soils in sorghum. MSc Thesis, College of Agriculture, Gilan University, Iran. (In Persian with English Abbstract)
Rahii A, Davvodi fard M, Azizi F and Habiby D, 2012. Effects of different amounts of humic acid and response curves in the Dactylis glomerata. Agriculture and Plant Breeding Journal 8(3): 28-15. (In Persian with English Abbstract)
Rauthan BS and Schnitzer M, 1986. Effects of soil fulvic acid on the growth and nutrient content of cucumber (Cucumus sativus) plants. Plant Soil 63: 491-495.
 Sabzavari S and  Khazaee HR, 2008. Effect of spraying various levels of humic acid on growth characteristics, yield and yield components of wheat (Triticum aestivum L.) Pishtaz cultivar. Agricultural Ecology 2 (1): 53-63. (In Persian with English Abbstract)
Salman SR, Abou-Hussein SD, Abdel-Mawgoud AMR and El-Nemr MA, 2005. Fruit yield and quality of watermelon as affected by hybrids and humic acid application. Journal of Applied Sciences Research 1: 51-58.
Sanchez Sanchez A, Sanchez anderu J, Juarez M, Jorda J and Bermudez D, 2002. Humic substances and amino acid improve effectiveness of Chelate FeEDDHA in lemons trees. Journal of Plant Nutrition 25(11): 2433-2442.
 Sanjari mijani M,  Cyrus Mehr AR and  Fakheri BA, 2015. Effect of drought stress and humic acid on some physiological properties of sour tea. Journal of Agriculture 2(17): 403-414. (In Persian with English Abbstract)
Schwertmann U, 1964. Differenzierung der eisenoxide des bodens durch extraktion mit ammoniumoxalat‐Lösung. Zeitschrift für Pflanzenernährung, Düngung, Bodenkunde 105(3): 194-202.
Shabazi K and Basharti H, 2013. Overview of fertility status of Iranian soil. Journal of Land Management 1(1): 1-17.  (In Persian with English Abbstract)
Shahandeh H, Hossner L and Turner F, 1994. A comparison of extraction methods for evaluating Fe and P in flooded rice soils. Plant and Soil 165 (2): 219-225.
Sharma BD, Arora H, Kumar R and Nayyar VK, 2004. Relationship between soil characteristics and total and DTPA-extractable micronutrients in Inceptisols of Punjab. Communications in Soil Science and Plant Analysis 35: 799–818.
Soltanpour PN and Schwab AP, 1977. A new soil test for simultaneous extraction of macro‐ and micro‐nutrients in alkaline soils. Communications in Soil Science and Plant Analysis 8(3): 195-207.
Therios I, Chouliaras V, Bizas D, Boukouvalas S, Palioura E, Hatzidimitriou E, Basioukas D, Gioldasis V and Stavrou N, 2005. Changes in leaf biochemical and physiological indices due to iron deficiency in citrus. AgroThesis 1: 18 – 25.
Trierweiler JF and Lindsay WL, 1969. EDTA-ammonium carbonate soil test for zine. Soil Science Society of America Journal 33(1): 49-54.
 Vatankhah A,  Mohammadkhani AR, Houshmand S and  Kiani Sh, 2015. Effect of humic acid and iron sulfate spraying on some physiological indices, quantity and quality of fruit grape varieties "Askari" cultivar. Production of Crop and Garden Cultivation 22(6): 107-119. (In Persian with English Abbstract)
Vempati RK and Loeppert RH, 1988. Chemistry and mineralogy of Fe- containing oxides and layer silicates in relation to plant available Fe. Journal of Plant Nutrition 11: 1557-1574.
Walkley A and Black IA, 1934. An examination of the Degtjareff method for determining soil organic matter, and a proposed modification of the chromic acid titration method. Soil Science 37(1):  29-38.
Wang L, Wu JP, Liu YX, Huang HQ and Fang QF, 2009. Spatial variability of micronutrients in rice grain and paddy soil. Pedosphere 19 (6): 748–755.
Wiren NV and Grusak MA, 2002. Summary of IX international symposium of iron nutrition and interaction in plants. Journal of Plant Nutrition 23: 2083-2102.
Xudan X, 1986. The effect of foliar application of fulvic acid on water use, nutrient uptake and wheat yield. Australian Journal of Agricultural Research 37: 343-350.
 
Water and Soil Science
Volume 28, Issue 4
January 2019
Pages 109-125

Files

Share

How to cite

Statistics