Interaction between Arbuscular Mycorrhizal Fungi and Iron Sources on Growth Parameters and Nutrients Uptake of Mexican Lime

Authors

1 Assoc. Prof., Dept. of Horticulture Science, Faculty of Agric., Shiraz Univ., Shiraz, Iran

2 2-Former M.Sc. Student, Dept. of Horticulture Science, Faculty of Agric., Shiraz Univ, Shiraz, Iran

3 Assoc. Prof., Dept. of Soil Science, Faculty of Agric., Shiraz Univ., Shiraz, Iran

4 Ph.D. Student, Dept. of Horticulture Science, Faculty of Agric., Shiraz Univ., Shiraz, Iran

Abstract

In order to the evaluation of the effect of mycorrhizal fungi, different sources and levels of iron on growth and nutrients uptake in Mexican lime (Citrus aurantifolia L) (one of the important rootstock in citrus growing regions in south of Iran), a greenhouse experiments were conducted with factorial arrangement in a completely randomized design with three factors and four replications. Treatments consisted of three levels of mycorhizal fungus (without fungal inoculation, inoculation with Rhizophagus intraradices, inoculation with Glomus versiforme), two sources of iron (iron chelate, iron solphate), and three iron levels (0, 7.5, 15 mgkg-1 Fe). The results indicated that co-application of mycorrhizal fungal species and iron sources was improved plant growth charactrices and the concentration of nitrogen, potassium, iron, zinc, copper, and manganese. In the treatments inoculated with mycorrhizal fungi, with an increase in iron levels, the nutrient concentrations of iron, zinc, copper and manganese in soil were increased, likely because of the balancing effect of mycorrhizal symbiosis on nutrient concentrations in plants. It seemed that co-application treatments of fungi with iron sulphate were effective on different growth indices of plant and could reduce iron chelate consumption or be used instead of it.

Keywords


 
Abbaspour H, SaeidSarand S, Afshar H. 2011. Improving drought tolerance of Pistaciavera L. seedlings by arbuscular mycorrhiza under greenhouse conditions. Journal of Medicinal Plants Research 5:7065-7072.
Andrade SA, Silveira PD, and Mazzefera P, 2010. Arbuscular mycorrhiza alters metal uptake and the physiological response of Coffea arabica seedlings to increasing Zn and Cu concentrations in soil. Science of the Total Environment 408: 5381-5391.
Auge RM, 2004. Arbuscular mycorrhizae and soil/plant water relations. Canadian Journal of Soil Science 84:373–8.
Bremner J M, 1996. Nitrogen-total. pp. 1085–1121. Sparks D L, (Ed.). In: Methods of Soil Analysis. Part 3. Chemical Methods. Soil Science Society of America. and American Society of Agronomy, Madison, WI.
Davies FT, Potter JR, and Linderman RG, 1993. Drought resistance of mycorrhizal pepper plants independent of leaf P concentration response in gas exchange and water relations. Physiologia Plantarum 87:45-53.
Desena J, Labate C A, and Cardoso EN, 2002. Micronutrient accumulation in mycorrhizal citrus under different phosphorus regimes. Acta Scientiarum. Maringá 24: 1265-1268.
El-shazly S, Abdel Naser M, Harhash M. 2000. Physiological and biochemical indices in Washington Novel orange trees as influenced by iron foliar application. Alexandria Journal of Agricultural Research 45:287-306.
Ferrol N, Barea JM, Azcon-Aguilar C, 2002. Mechanisms of nutrient transport across interfaces in arbuscular mycorrhizas. Plant and Soil 244(1-2): 231-237.
Fotouhi Ghazvini R, 1999. Citrus Growing in Iran. Published by University of Guilan, Iran. 158 pp.
Gee GW, and Bauder JW, 1986. Particle size analysis. A. Klute (ed.), Methods of Soil Analysis. 9(1): 383-411. Part 1. Physical and Mineralogical Methods. 2nd Edition, American Society of Agronomy, Madison, WI.
Graham JH, and Syvertsen JP,1985. Host determinants of mycorrhizal dependency of citrus rootstock seedlings. New Phytologist 101:667-676.
Graham H, and Syvertsen JP,1984. Infuence of vesicular–arbuscular mycorrhiza on the hydraulic conductivity of roots of two citrus rootstocks. New Phytologist 97: 277–284.
Gosling P, Hodge A, Goodless G, and Bending G D, 2006. Arbuscular mycorrhizal fungi and organic farming. Agriculture, Ecosystems and Environment 113: 17–35.
Haghighatnia H, Nadian HA, and Rejali F, 2011. Effects of mycorrhizal colonization on growth, nutrients uptake and some other characteristics of Citrus volkameriana rootstock under drought stress. World Applied Sciences Journal 13 (5):1077-1084.
Khade SH, Wand Bernard F R, 2009. Studies on effects of Arbuscular mycorrhizal (am.) fungi on mineral nutrition of Carica papaya L.  NotulaeBotanicae Horti Agrobotanici. Cluj 37 (1):183-186.
Kormanik P P, and McGraw AC, 1982. Quantification of vesicular-arbuscular mycorrhizae in plant root. In: Methods and Principles of Mycorrhizal Reseach, ­ (Ed.  by N. C.  Schenck (, pp. 37-45. The American Phytopathological Society, St. Paul, Minnesota.
Knudsen D, Peterson GA, and Pratt PF, 1982. Lithium, Sodium and Potassium. pp. 225-246. In: AL Page et al (eds). Methods of Soil Analysis, Part 2. American Society of Agronomy, Madison.
Kuo S, 1996. Phosphorus. In Methods of Soil Analysis. Part 3, Chemical Methods; Sparks, Madison, Wisconsin,869–919.
Lopez-Bucio J, Cruz-Ramirez A, Herrera-Estrella L, 2003. The role of nutrient availability in regulating root architecture. Current Opinion in Plant Biology 6:280–287.
Lindsay WL, and Norvell WA, 1978. Development of a DTPA soil test for zinc, iron, manganese, and copper. Soil Science Society of American Journal 42: 241-428.
Mukerji, KG, and Chamola BP, 2003. Compendium of Mycorrhizal Research. A. P. H. Publisher, New Delhi. 310 pp.
Nelson DW and Sommers LE, 1996. Total carbon, organic carbon and organic matter. In: Method of Soil Analysis. Part III. Chemical Methods. WI. pp. 961-1010. Sparks DL (Ed). Soil Science Society of America Journal and American Society of Agronomy‚ Madison.
Nogueira MA, and Cardoso EB, 2006. Plant growth and phosphorus uptake in mycorrhizal rangpour lime seedling under different levels of phosphorous. Bras. Brasilia, 41:93-99.
Olsen SR‚ Cole CV‚ Watanabe FS and Dean LA, 1954. Estimation of Available Phosphorus in Soils by Extraction with Sodium Bicarbonate. United states Department of Agriculture. Circular 939.
Ortas I, Ortakçi D, Kaya Z, Cinarand A, Onelge N, 2006. Mycorrizal dependency of sour orange in relation to nitrogen and zinc nutriation. Journal of Plant Nutrition 25(6):1263-1279.
Perez-Perez JM., 2007. Hormone signaling and root development: an update on the latest Arabidopsis thaliana research. Funct. Plant Biology 34:163–171.
Pixão CM, Oliveira A R and Amoria RTD, 2007. Arbuscular mycorrhizal fungi effect on growth and nutrition of citrus rootstock. Magistra 19: 47-59.
Rhoades JD, 1996. Salinity: Electrical conductivity and total Dissolved Solids‚ Madison WI.417-435. Sparks D L (Ed).  In: Methods of Soil Analysis‚ Part 3. chemical Methods. Soil Science Society of America, and American Society of Agronomy.
Salehi F, Moradi Ghahdrijani M, Mirabolfathi M and Aliasgharzadeh N, 2008. Effect of VA mycorrhizal colonization and different levels of phosphorus on P, K, Ca, Mg and Zn uptake and vegetative traits of pistachio. Pajouhesh va Sazandgi, 20(4): 48-56.
Smith SE and Read DJ, 1997.  Mycorrhizal Symbiosis. San Diego:  Academic Press, 815 pp.
Syvertsen J P and Graham JH, 1990. Influence of vesicular arbuscular mycorrhizae and leaf age on net gas exchange of citrus leaves. Plant Physiology 94: 1424-1428.
Tang M, Chen H, Huang JC and Tian ZQ, 2009. Arbuscular mycorrhiza fungi effects on the growth and physiology of (Zea mays L.) seedlings under diesel stress. Soil Biology and Biochemistry 41: 936–940.
Thomas GW, 1996. Soil pH and Soil Acidity. Madison, WI. pp. 475-490.
Wang M, Christie P, Xiao Z, Wang P, Lio J, and Xia R, 2008. Arbuscular mycorrhizal enhancement of iron concentration by Poncirus trifoliata L. Raf and Citrus reticulata Blanco grown on sand medium under different pH. Biology and Fertility of Soils 45:65-72.
Wu Q S, and Xia R X and Zou Y N, 2006. Reactive oxygen metabolism in nonmycorrhizal citrus (Poncirus trifoliata) seedlings subjected to water stress. Journal of Plant Physiology 163:1101-1110.
Wu QS and Xia RX, 2006.  Arbuscular mycorrhizal fungi influence growth, osmotic adjustment and photosynthesis of citrus under well-watered and water stress conditions. Journal of Plant Physiology 163:417-425.
Wu Q S, Xia RX and Zou YN, 2008. Improved soil structure and citrus growth after inoculation with three arbuscular mycorrhizal fungi under drought stress. European Journal of Soil Biology 44(1):122-128.
Wu QS, and Zou YN, 2009. The effect of dual application of arbuscular mycorrhizal fungi and polyamin upon growth and nutrient uptake of trifoliate orange seedling. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 37 (2):95-98.
Wu QS, Zou YN, He X, and Luo P, 2011. Arbuscular mycorrhizal fungi can alter some root characters and physiological status in trifoliate orange (Poncirus trifoliata L. Raf.) seedlings. Plant Growth Regulation 65:273-278.
Yao Q, Zhu H H, Chen JZ, 2005. Growth responses and endogenous IAA and iPAs changes of litchi (Litchi chinensis Sonn.) seedlings induced by arbuscular mycorrhizal fungal inoculation. Horticultural Science 105(1):145–151.