Inoculation Effect of Some Liquid Inocula of Enterobacter cloacae on Oil and Fatty Acids Percentages, and Nutritional Indices of Rapeseed (Brassica napus L.)

Authors

1 Department of Soil Science, University of Tabriz

2 Scientific staff of University of Tabriz

3 Soil Science Department, University of Tabriz

Abstract

Background and Objectives: Biofertilizers play major role in sustainable agriculture. Biofertilizers are inoculants containing useful microorganisms which are used in solid, liquid and/or encapsulated formulations. Different materials can be used as liquid carriers. Increasing inoculant longevity by using different materials is the main purpose in production of liquid inocula. These additives are carbon-based and reduce environmental stresses. In this study the effectiveness of liquid inocula of Enterobacter cloacae S16-3 on oil content, fatty acids and nutrient uptake of rapeseed in a sterile sandy loam soil were evaluated. Nine liquid inocula of S16-3 (F1-F9) were prepared using different amounts of materials including glycerol, polyethylene glycol (PEG), trehalose, carboxymethylcellulose (CMC), Arabic gum (AG), polyvinylpyrrolidone (PVP), glucose and starch, in different combinations. Then, the effect of liquid inocula on growth of rapeseed (Brassica napus L.) cultivar hyola 308 was investigated.
Methodology: The experiment was carried out in greenhouse condition based on completely randomized design (CRD) with three replications. The factors were including 9 liquid inocula (F1-F9), in all these treatments, 70% of NPK was added to the soil in each pot, since we assumed that S16-3 is able to supply only 30% of these elements, one control treatment without adding any bacteria and fertilizer (negative control), and two positive controls (using NPK equal to 70% and 100% of fertilizer recommendation). Germinated seedlings of rapeseed were planted and inoculated with inocula in each pot. Except for NPK elements, other micronutrients were provided based on soil test; however, in the case of nitrogen, phosphorus and potassium elements that were the main objective of this experiment, no chemical fertilizer and bacteria were used in negative control treatment. In positive control (100NPK), based on the soil test and previous experiments, 100% of the recommended amount of fertilizer, equivalent (56.5 mg N/kg soil, from Urea source), (13 mg P/kg soil, from triple superphosphate source) and (31.3 mg K/kg soil, from potassium sulfate source) were used, moreover, for other positive control treatment (70NPK), 70% of the above values in pot culture were added to the pots. During the growth period, irrigation was performed with sterile water and the moisture content of the pots was maintained by weighing in a moisture range (0.7 - 0.8 FC). In pot culture, characteristics such as dry weight of roots, shoots and seed, seed oil percentage and fatty acids, uptake of nitrogen, phosphorus, potassium, iron, zinc, manganese and calcium in the root and shoot were measured.
Findings: The results obtained from the greenhouse experiments showed that the dry weight of roots, shoots and seed, seed oil percentage and fatty acids, uptake of nitrogen, phosphorus, potassium, iron, zinc, manganese and calcium in the root and shoot of rapeseed (Brassica napus L.) cultivar hyola 308 were significantly influenced by the presence of E. cloacae bacteria (in the form of nine inocula) and 100NPK and 70NPK treatments. The highest percentage of seed oil (47.02%) and the highest amount of oleic acid (53.1%) was obtained by F9 (glycerol, glucose, AG, PEG) treatment, and oil quality was affected by bacterial inoculation. the highest amount of saturated fatty acids, such as stearic acid (4.5%) and palmitic acid (5.6%) was measured in without inoculation treatment (negative control). Nutrition analysis in dry tissue of plant showed that 100NPK treatment had the highest N, K, Fe, Zn and Ca uptake in the plant, and among liquid inocula treatments (F1-F9), the highest uptake of these elements belonged to the F5 (AG, starch, PEG). The highest amount of total P in F1 treatment (glycerol, trehalose, CMC) and F4 (trehalose, AG, PEG) and highest Mn absorbed in the plant were obtained in F5 treatment.
Conclusion: In most measured indices, the effects of liquid inocula had higher performance than without inoculation treatment (negative control). From the nutritional point of view, F5 (AG, starch, PEG) liquid inoculant were better than the other inocula. F9 treatment had a significant effect on quantitative and qualitative characteristics of rapeseed oil. . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Keywords

References

Abdul M and Fayyazul H, 2006. Effects of sulphur on fatty acid accumulation in Brassica cultivars. International Journal of Agricultural and Biological
 
5: 588-592.
Ahmad A and Abdin MZ, 2000. Effect of sulphur application on lipid, RNA and fatty acid content in developing seeds of rapeseed (Brassica campestris L.). Plant Sciences 150: 71-76.
Ahmad F, Ahmad I and Khan MS, 2008. Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiological Research 163 (2):173-181.
Ahmad G, Jan A, Arif M, Jan MT and Khattak RA, 2007. Influence of nitrogen and sulfur fertilization on quality of canola (Brassica napus L.) under rainfed conditions. Journal of Zhejiang University-SCIENCE B 8 (10): 731-737.
Akbari P, Ghalavand A and Modarres Sanavy SAM, 2010. Effects of different nutrition systems and biofertilizer (PGPR) on phenology period yield and yield components of sunflower (Helianthus annuus L.). Journal of Crop Production. 2 (3): 119-134. (In Persian with English abstract).
Alipour ZT and Sobhanipour A, 2012. The Effect of Thiobacillus and Pseudomonas fluorescens inoculation on maize growth and Fe uptake. Annals of Biological Research, 3: 1661-1666.
Azadmard Damirchi S, 2012. Food Chemistry and Decomposition. Publication of Amidi. (In Persian with English abstract).
Barker AV and Pilbeam DJ, 2006. Handbook of Plant Nutrition. CRC Press, 196 p.
Besharati H, Pashapour S and Rezazadeh M, 2017. The evaluation of plant growth promoting rhizobacteria effect for improving soybean growth indices. Iranian Journal of Field Crop Science 47(4): 671-687. (In Persian with English abstract).
Dong H, 2010. Mineral-microbe interactions: a review. Frontiers of Earth Science in China 4 (2):127-147.
Fieldsend JK, Murray FE, Bilsborrow PE, Milford GFJ and Evans EJ, 1991. Glucosinolate accumulation during seed development in winter sown oilseed rape (B. napus). Proceedings of the Eighth International Rapeseed Congress, July 9 to 11, Saskatoon, Canada. 686-694.
Flagella Z, Rotunnon T, Tarantino E, Di-Caterina R and Decaro A, 2002. Changes in seed yield and oil fatty acid composition of high oleic sunflower (Helianthus annus L.) hybrids in relation to sowing date and water regime. European Journal of Agronomy 17: 3. 221-230.
Gao J, Thelen KD, Min DH, Smith S and Hao X, 2010. Effects of manure and fertilizer applications on canola oil content and fatty acid composition. Journal of Crop and Soil Science 102 (2): 790-797.
Ghadamkhani A, Enayatizamir N and Norouzi Masir M, 2018. Effect of plant growth promoting bacteria on soil available iron and its uptake by wheat. Journal of Agricultural Science and Sustainable Production. 28(2): 53-64. (In Persian with English abstract).
Ghasemi Piranlo F, 2018. The efficiency of several solid and liquid carriers to increase the survival of Enterobacter cloacae, Iran. MSc. thesis Faculty of Agriculture, University of Tabriz. (In Persian with English abstract).
Ghasemi Piranlo F, Sarikhani MR and Najafi N, 2019. Study the survival of Enterobacter cloacae bacterium in several solid carriers and effect of prepared inoculants on ggermination and growth of wheat. Journal of Agricultural Science and Sustainable Production. 29 (3): 167-180. (In Persian with English abstract).
Hu Y, Ye X, Shi L, Duan H and Xu F, 2010. Genotypic differences in root morphology and phosphorus uptake kinetics in Brassica napus under low phosphorus supply. Journal of Plant Nutrition. 33 (6): 889-901.
Jones JR, 2001. Laboratory Guide for Conducting Soil Tests and Plant Analysis, CRC press.
Kalra YP, 1998. Handbook of Reference Methods for Plant Analysis. CRC Press, USA.
Karlidag H, Estiken A, Turan M and Sahin F, 2007. Effects of root inoculation of plant growth promoting rhizobacteria (PGPR) on yield, growth and nutrient element contents of leaves of apple. Science Horticulturae 114: 16–20.
Kazemi Oskuei B, Bandehagh A, Sarikhani MR and Komatsu S, 2018. Protein profiles underlying the effect of plant growth promoting rhizobacteria on Canola under osmotic stress. Journal of Plant Growth Regulation 37 (2): 560-574.
Khajehpour MR, 2005. Industrial Crops. Jahade-e-Daneshghahi Isfahan Press. (In Persian).
Khaliq A, 2004. Irrigation and nitrogen management effects on productivity of hybrid sunflower (Helianthus annuus L.). Ph.D. thesis, Department of Agronomy, University of Agriculture, Faisalabad, Pakistan.
Lickfett TB, Matthaus Velasco L and Mollers C, 1999. Seed yield, oil and phytate concentration in the seeds of two oilseed rape cultivars as affected by different phosphorus supply. European Journal of Agronomy 11: 293-299.
Mashhadi Asghari S and Aliasgharzadeh N, 2005. Comparison of five carriers of Sinorhizobium meliloti to produce alfalfa inoculant. Water and Soil Sciences. 8(4): 63-75. (In Persian with English abstract). 
Mohamadvarzi R, Habibi D, Vazan S and Pazoki AR, 2011. Effect of plants growth promoting rhizobacteria and nitrogen fertilizer on yield and yield components of sunflower. Pp. 1-11. 5th National Conference on New Ideas in Agriculture 27-28 January, Esfahan, Iran. (In Persian with English abstract).
Mohamadzadeh J, 2015. Investigation of the effect of enzymatic and solvent extraction methods on the quality characteristics of oilseed rape and protein. Journal of Iranian Oilseed Plants 4(1): 23-32. (In Persian with English abstract). 
Mohammadi T, Azizi MH and Taslimi A, 2007. Relation of fatty acids composition with stability of sunflower and canola oil blends. Journal of Food Science and Technology 4: 67–76.
Mohammadzadeh J and Yaghbani M, 2005. Effect of harvest time moisture and drying temperature of rapeseed on the quality of extracted oil. Journal of Agricultural Sciences and Natural Resources 12 (5): 119-127.
Moller C and Schierholt A, 2002. Genetic variation of palmitate and oil content in a winter oilseed rape doubled haploid population segregating for oleat content. Crop Science 42: 379-384.
Moradi Sh and Sarikhani MR, 2016. Comparison of dissolution of phosphate from sources of phosphate rock and Tricalcium phosphate by some phosphate solubilizing bacteria. Pp. 1-6. 2th National Congress for the Development of Agricultural Science and Natural Resources, 22 April, Gorgan, Iran. (In Persian with English abstract).
Naseri F, 1991. Oil Seeds. Publication of Astan Qods Razavi, Mashhad. (In Persian). 
Nosheen A, Bano A and Ullah F, 2013. The role of plant growh promoting rhizobacteria on oil yield and biodiesel production of canola (Brassica napus L.). Energy Sources 35: 1574-1581.
Olsen SR and Sommers LE, 1982. Phosphorus. In Klute A, (Ed.), Pp. 403-427. Methods of Soil Analysis Part.1 Chemical and Biological Properties. Soil Science Society of America, Madison, WI.
Panlada T, Payakapong PW and Boonkerd N, 2007. Growth, survival and field performance of Bradyrhizobial liquid inoculant formulations with polymeric additives. Science Asia 33: 69-77.
Paul EA, 2007. Soil Microbiology, Ecology and Biochemistry. 3rd Edition. Linacre House, Jordan Hill, Oxford OX2, 8DP,UK.
Ramesh A, Sharma SK, Sharma MP, Yadav N and Joshi OP, 2014. Plant growth promoting traits in Enterobacter cloacae subsp. dissolvens MDSR9 isolated from soybean rhizosphere and its impact on growth and nutrition of soybean and wheat upon inoculation. Agricultural Research 31: 53–66.
Sajedi NA, Madani H and Mirzakhani M, 2011. Evaluation of bio-chemical fertilizers on agronomical traits and oil percentage in sunflower. New Finding in Agriculture 5(4): 377-387. (In Persian with English abstract).
Sarikhani MR, Aliasgharzad N and Khooshroo B, 2018. Effectiveness study of phosphate solubilizing bacteria in the formulation of phosphatic microbial fertilizers on corn. Iranian Journal of Soil and Water Research. 49(1): 71-81. (In Persian with English abstract).
Sarikhani MR, Oustan S, Ebrahimi M and Aliasgharzad N, 2018. Isolation and identification of potassium releasing bacteria in soil and assessment of their ability to release potassium for plants. European Journal of Soil Science 69(6): 1078-1086.
Sayed AV and Hossein AF, 2011. Investigation of biofertilizers influence on quantity and quality characteristics in Nigella sativa L. Horticulture 3(3): 88–92.
Seyed Sharifi R, 2016. Aplication of biofertilizers and zinc increases yield, nodulation and unsaturated fatty acids of soybean. Zemdirbyste- Agriculture, 103 (3): 251-258.
Shahidi M, Hameed S, Imran A, Ali S and Elsas JD, 2012. Root colonization and growth promotion of sunflower (Helianthus annuus L.) by phosphate solubilizing Enterobacter sp. Fs-11. World Journal of Microbiology and Biotechnology 28:2749–2758.
Shankar M, Ponraj P, Ilakkiam D and Gunasekaran P, 2011. Root colonization of a rice growth promoting strain of Enterobacter cloacae. Journal of Basic Microbiology 51: 523–530.
Shehata MM and EL-Khawas SA, 2003. Effect of two biofertilizers on growth parameters, yield characters, nitrogenous components, nucleic acids content, minerals, oil content, protein profiles and DNA banding pattern of sunflower yield. Pakistan Journal of Biological Sciences 6 (14): 1257-1268.
Spaepen S, Dobbelaere S, Croonenborghs A and Vanderleyden J, 2008. Effects of Azospirillum brasilense indole-3-acetic acid production on inoculated wheat plants. Plant and Soil 312: 15-23.
Steer BT and Seiler GI, 1990. Changes in fatty acid composition of sunflower (Helianthus annuus L.) seeds in response to time of nitrogen application, supply rates and defoliation. Journal of the Science of Food and Agriculture 51: 11-26.
Tittabutr P, 2005. Development of rhizobial liquid inoculant production. Ph.D Dissertation in Biotechnology, Suranaree University of Technology, 228p.
Westerman RL, 1990. Soil Testing and Plant Analysis. 3rd Edition, Book Series No. 3, SSSA, USA.
Yao L, Wu Z, Zheng Y, Kaleem I and Li C, 2010. Growth promotion and protection against salt stress by Pseudomonas putida Rs-198 on cotton. European Journal of Soil Biology 46: 49-54.
Water and Soil Science
Volume 34, Issue 2
June 2024
Pages 17-38

Files

Share

How to cite

Statistics