Comparison of Organic Acids and Chelates for Enhancing Phytoremediation of Lead from a Contaminated Soil

Authors

1 Associate Professor, Department of Agronomy, Payame Noor University, Iran

2 PhD student in soil chemistry, Urmia University and Lecturer in Payame Noor University, Iran

3 MSc. in Agronomy, Gorgan University of Agricultural Sciences and Natural Resources, Iran

Abstract

Phytoremediation is a method for decreasing lead contamination in soil and the use of chelates increases the efficiency of this method. In this study, phytoremediation efficiency of savory plant (Satureja hortensis L.) to remove lead from contaminated soil was investigated in the presence of chelates including ethylene-diamine tetra-acetic acid (EDTA) and diethylene diamine penta-acetic acid (DTPA) and organic acids including acetic acid (AA), citric acid (CA) and tartaric acid (TA) at concentrations of 0 and 10 mmol kg-1. The soil was amended with five levels of 0, 100, 200, 300 and 400 mg kg-1 lead from lead nitrate source. The experiment was conducted as factorial based on completely randomized design with three replications. The results showed that the shoot dry weight was higher in organic acids treatments than synthetic chelates, and increasing of lead concentration up to 300 and 400 mg kg-1 decreased shoot dry weight. Acetic acid significantly increased the available lead concentration in the soil and the highest amount was obtained at level of 400 mg kg-1 lead in soil. The highest plant extraction potential was observed with acetic acid at 100 mg kg-1. Organic acids showed more effective role in increasing the extraction of lead compared to the than chelates. The results of this study showed that Satureja hortensis had high ability to extract lead from soil at Pb levels of up to 200 mg kg-1. Due to the high translocation efficiency of lead from root to shoot, this plant can be used for soil remediation with application of organic acids.

Keywords


Al-Busaidi P, Cookson L and Yamamoto T, 2005. Methods of pH determination in calcareous soil: use of electrolytes and suspension effect. Soil Research 43: 541-545.
Arabi Z, Homaee M and Asadi M, 2011. Comparison effects of citric acid and synthetic chelators in enhancing phytoremediation of cadmium. Journal of Science and Technology of Agriculture and Natural Resources 14: 85-95.(In Persian with English abstract).
Babaeian E and Homaei M, 2011. Enhancing lead phytoextraction of land cress (Barbara verna) using aminopolycarboxylic acids. Journal of Water and Soil 24: 1142-1150. (In Persian with English abstract).
Babaeian E, Homaei M and Rahnemaie R, 2012. Enhancing phytoextraction of lead contaminated soils by carrot (Daucus carrota) using synthetic and natural chelates. Journal of Water and Soil 26: 607-618. (In Persian with English abstract).
Blaylock MJ, Salt DE, Dushenkov Zhakarova O, Gussman C, Kapulnik Y, Ensley BD and Raskin I, 1997. Enhanced accumulation of Pb in Indian mustard by soil-applied chelating agents. Environmental Science and Technology 31(3): 860–865.
Bouyoucos GJ, 1936. Direction for making mechanical analysis of soil by the hydrometer method. Journal of Soil Science 41: 225-228.
Bremner JM and Mulvaney C. 1982. Nitrogen Total. Pp. 595-624. In: Page AL, Miller RH and Keeney DR (Eds). Methods of Soil Analysis. Part 2. Chemical and Microbiological Properties. Soil Science Society of America, Madison, Wisconsin.
Bruckner MZ, 2012. Water and Soil Characterization, pH and Electrical Conductivity. Microbial Life Educational Resources, Montana State University Bozeman.
Chand V and Prasad S, 2013. ICP-OES assessment of heavy metal contamination in tropical marine sediments: a comparative study of two digestion techniques. Microchemical Journal 111:53-61.
Chao JC, Hong A, Okey RW and Peters RW, 1998. Selection of chelating agents for remediation of radionuclide-contaminated soil. Pp.142-155, Proceedings of the Conference on Hazardous Waste Research.
Chen Y, Xiangdong L and Shen Z, 2004. Leaching and uptake of heavy metals by ten different species of plants during an EDTA assisted phytoextraction process. Chemosphere 57: 187-196.
Dinkelaker B, Römheld V and Marschner H, 1989. Citric acid excretion and precipitation of calcium citrate in the rhizosphere of white lupin (Lupinus albus L.). Journal Plant, Cell and Environment 12: 285-292.
Do Nascimento CA, Amarasiriwardena D And Xing B, 2006. Comparison of natural organic acids and synthetic chelates at enhancing phytoextraction of metals from a multi-metal contaminated soil. Journal Environmental Pollution 140: 114-123.
Dodge CJ and Francis AJ, 1994. Photodegradation of uranium-citrate complex with uranium recovery. Environmental Science and Technology 28: 1300-1306.
Evangelou MW, Ebel M and Schaeffer A, 2006. Evaluation of the effect of small organic acids on phytoextraction of Cu and Pb from soil with tobacco Nicotiana tabacum. Chemosphere 63: 996-1004.
Fatahi Kiasari EF, Fotovat A, Astaraei AR and Haghnia GH, 2010. Lead phytoextraction from soil by corn, sunflower, and cotton applying EDTA and sulfuric acid. Journal of Science and Technology of Agriculture and Natural Resource 14: 57-69. (In Persian with English abstract).
Gee GW and Bauder JW, 1986. Particle analysis In: A. Klute (Ed.), Method of Soils Analysis. Part 2, Physical and Mineralogical Methods. Soil Science Society America, Madison, WI.
Gzar HA, Abdul-Hameed, AS and Yahya AY, 2014. Extraction of lead, cadmium and nickel from contaminated soil using acetic acid. Open Journal of Soil Science 4: 207-210.
Huang JW, Chen J, Berti WR and Cunningham SD, 1997. Phytoremediation of lead-contaminated soils: role of synthetic chelates in lead phytoextraction. Environmental Science and Technology 31: 800-805.
Kabata-Pendias A, 2004. Soil–plant transfer of trace elements an environmental issue. Geoderma 122: 143-149.
 Karczewska A, Orlow K, Kabala C, Szopka K and Galka B, 2011. Effects of chelating compounds on mobilization and phytoextraction of copper and lead in contaminated soils. Communications in Soil Science and Plant Analysis 42: 1379-1389.
Kramer U, Pickering IJ, Prince RC, Raskin IL and Salt DE, 2000. Subcellular localization and speciation of nickel in hyperaccumulator and non-accumulator Thlaspi species. Plant Physiology 122: 1343-1353.
 Liu D, Islam E, Li T, Yang X, Jin X and Mahmood Q, 2008. Comparison of synthetic chelators and low molecular weight organic acids in enhancing phytoextraction of heavy metals by two ecotypes of Sedum alfredii hance. Journal of Hazardous Materials 153: 114-122.
Loeppert RH and Suars DL, 1996. Carbonate and Gypsum. Pp. 437-474. In: Sparks DL, (Ed.), Method of Soils Analysis. Part 3. Chemical Methods. Soil Science Society of America, Madison, WI.
Lone MI, He ZL, Stoffella PJ and Yang XE, 2008. Phytoremediation of heavy metal polluted soils and water: progresses and perspectives. Journal of Zhejiang University Science B 9: 210-220.
Madrid F, Liphadzi MS and Kirkham MB, 2003. Heavy metal displacement in chelate-irrigated soil during phytoremediation. Journal of Hydrology 271: 107-119.
Mohamadian Z, Golamalizadeh A, Gorbani M and Mohkami Z, 2015. The effect of potassium fertilizers on lead and cadmium phytoremediation by lavender (Lavendula officnalis) in a polluted soil. Journal Water and Soil Conservation 23: 273-287. (In Persian with English abstract).
Muhammad D, Chen F, Zhao J, Zhang G and Wu F, 2009. Comparison of EDTA-and citric acid-enhanced phytoextraction of heavy metals in artificially metal contaminated soil by Typha angustifolia. International Journal of Phytoremediation 11: 558-574.      
Naderi M, Danesh Shahraki A and Naderi R, 2011. Review on phytoremediation of contaminated soil by heavy metal. Journal of Human and Environment 23: 35-49. (In Persian with English abstract).
Neisi A, Vosoughi M, Mohammadi MJ, Mohammadi B and Naeimabadi A, 2014. Phytoremediation by helianthus plant. Journal of Torbat Heydariyeh University of Medical Sciences 2: 55-65. (In Persian with English abstract).
Quartacci MF, Baker AJM and Navari-Izzo F, 2005. Nitrilotriacetate-and citric acid-assisted phytoextraction of cadmium by Indian mustard (Brassica juncea L. Czernj). Chemosphere 59: 1249-1255.
Sabir M, Waraich EA, Hakeem KR, Öztürk M, Ahmad HR and Shahid M, 2015. Phytoremediation: Mechanisms and adaptations. Soil Remediat Plants 4:85–105.
Shu WS, Ye ZH, Lan CY, Zhang ZQ and Wong MH, 2001. Acidification of lead/zinc mine tailings and its effect on heavy metal mobility. Environment International 26: 389-394.
Terry N and Ban Uelos G, 2000. Phytoremediation of Contaminated Soil and Water. Lewis Publishers, Boca Raton, FL.
 Turgut C, Pepe MK and Cutright TJ, 2004. The effect of EDTA and citric acid on phytoremediation of Cd, Cr, and Ni from soil using Helianthus annuus. Environmental Pollution 131: 147-154.
Vassil AD, Kapulnik Y, Raskin I and Salt DE, 1998. The role of EDTA in lead transport and accumulation by Indian mustard. Plant Physiology 117: 447-453.
Walker DJ, Clemente R and Bernal MP, 2004. Contrasting effects of manure and compost on soil pH, heavy metal availability and growth of Chenopodium album L. in a soil contaminated by pyritic mine waste. Chemosphere 57: 215-224.
Walkley A, 1947. Organic carbon by the Walkley-Black oxidation procedure. Soil Science 63: 251-264.
Wu LH, Luo YM, Xing XR and Christie P, 2004. EDTA-enhanced phytoremediation of heavy metal contaminated soil with Indian mustard and associated potential leaching risk. Agriculture. Ecosystems and Environment 102: 307-318.