Efficiency of Bacillus and Pseudomonas Bacteria in Bioremediation of a Soil Contaminated with Hydrocarbons

Document Type : Research Paper

Authors

Abstract

       Soil contamination with petroleum compounds since the petroleum exploration in the last century in Iran, and accumulation of these compounds in the environment threaten the health of soil and water resources of the country. The current research was conducted with the purpose of reaching to an efficient approach for the remediation of soils polluted with petroleum compounds. In this research the effect of five experimental treatments including various species of Bacillus and Pseudomonas bacteria with and without barely (Karron cv.) planting on the bio-degradation of those compounds in an artificially polluted loam soil was investigated. Analysis of the data indicated that the maximum purification belonged to the Bacillus 2 treatment. With barely planting this bacteria reduced soil total petroleum hydrocarbons up to %72.5 during 105 days. Without barely the reduction became %59.8 at the same period. The rate of bio-degradation was also highest at the same treatment, meaning that during a 20-day the reduction of catumination became %40. Degredation rate considerably increased with the presence of bacteria in the soil comparing to the barely planting alone. Inoculating of contaminated soil with bacteria without the need for particular nursery seems to be enough to control effectively the soil pollution. Among the several examined bacteria, Bacillus 2 turned to be the most suitable one for removing the petroleum- loased contamination. 

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شهریاری م، 1385. تاثیر مخلوط دو گیاه فسکیو و یونجه در گیاه­پالایی خاک آلوده به نفت خام، نشریه علوم محیطی، شماره 13. صفحه­های 33-40.
عنایت زاده م، 1385. حذف آلودگی­های حاصل از نفت خام در محیط زیست و آب­های آلوده به روش زیستی، پایان نامه کارشناسی ارشد، گروه بیوتکنولوژی دانشگاه صنعتی امیرکبیر.
BalajiMB, Fengxiang HX, Susan DV, David ML and Yi Su, 2007.Effects of Zn and Cd accumulation on structural and physiological characteristics of barley plants.  Braz  J  Plant Physiol 19:15-22. 
Boossert ID and Bartha R, 1984. The fate of  petroleum in soil ecosystems. Pp. 435-474. In: Atlas RM (ed). Petroleum Microbiology.Mac Millan Publishing Co, New York.
Das K and MukherjeeAK, 2006. Crude petroleum-oil biodegradation efficiency of Bacillus subtilis and Pseudomonas aeruginosa strains isolated from a petroleum-oil contaminated soil from north-east India. Bioresource Technology 98: 1339-1345.
Frick CM, Farrell RE and Germida JJ, 1999. Assessment of Phytoremediation as an In-Situ Technique for Cleaning Oil- Contaminated Sites. Saskatchewan, Canada.
Hutchinson SL, Schwab AP and Banks MK, 2001. Phytoremediation of aged petroleum sludge: Effect ofirrigation techniques and scheduling. Journal of Environmental Quality 30:1516-1522.
Houba VJG, Van der Lee JJ, Navozamasky I and Walgina I, 1989. Soil and Plant Analysis, a series of syllabi. Soil Analysis Procedures. Soil Sci 13:487-496.
Kim MH and Hao OJ, 1999. Co-metabolic degradation of chlorophenols by Acinetobacterspecies. Water Res 33: 562-574.
Klute A(ed.), 1986. Methods of Soil Analysis.Part 1, 2nd ed. American Society of Agronomy and Soil Science Society of America, Madison, WI.
Lee K and Gibson DT, 1996. Toluene and ethylbenzene oxidation by purified naphtalene dioxygenase from Pseudomonas sp. Strain NCIB 9816-4. Appl Environ Microbiol 62: 3101-3106.
Lee K, Brand JM and Gibson DT, 1995. Stereospecific sulfoxidation by toluene and naphtalene dioxygenases. Biochem Biophys Res Commun212: 9-15.
Schwab AP, Banks MK and Arunachalam M, 1995. Biodegradation of Polycyclic Aromatic hydrocarbons in Rhizosphere Soil. Bioremediation of Recalcitrant Organics. Battelle Press, Columbus. OH.
Shields MS, Montgomery SO, Cuskey SM Chapman PJ and Priichard PH, 1991. Mutants of Pseudomonas cepacia G4 defective in catabolism of aromatic compounds and trichloroethylene. Appl Environ Microbiol57: 1935-1941.
Wackett LP, BrusseauGA, Householder SR and Hanson RS, 1989. A Survey of microbial oxygenases: trichloroethylene degradation by propane-oxidizing bacteria. Appl Environ Microbiol55: 2960-2964.
Walkley A and BlackIA, 1934. An examination of Degtjareff method for determining soil organic matter and a proposed modification of the chromic acid totration method. Soil Sci 37: 29-37.