Evaluation of Plant Growth Promoting Characteristics in Commercial Biofertilizers of Azotobarvar, Potabarvar, Ruein and Phosco

Document Type : Research Paper

Authors

1 PhD Student of Soil Biology and Biotechnology, Faculty of Agriculture, Univ. of Tabriz, Iran

2 Assoc. Prof. of Soil Biology and Biotechnology, Dept. of Soil Science, Univ. of Tabriz, Iran

Abstract

In this study, three domestic biofertilizer samples including Azotobarvar, Potabarvar, Ruein, and a sample of Indian biofertilizer called Phosco were studied in terms of microbial population count and plant growth promoting characteristics. The results of plate counting revealed that Potabarvar had no alive bacterial population, but the number of viable cells of Azotobarvar, Ruein and Phosco were 3.5 × 108, 5.2 × 108 and 6.9 × 103 CFU g-1, respectively. Moreover, two isolates (Az1 and Az2), three isolates (R1, R2 and R3) and three isolates (P1, P2 and P3) were isolated in Azotobarvar, Ruein and Phosco, respectively. The in-vitro results of plant growth promoting characteristics showed that in the solid LG medium R1 and Az2 and in the liquid LG medium, P1, R1 and Az2 and had the highest nitrogen fixation, respectively. In phosphate solubilizing from tricalcium phosphate, Az1 and Az2, in potassium releasing ability, Az1 and P1 isolates from biotite and Az1 and P2 from muscovite and in production of auxin P1 in the presence of tryptophan had the highest performance, respectively. The isolates P1, P3, R1 and R3 had antifungal activity against Fusarium solani. Summarizing the results of microbial population counts as well as plant growth promoting characteristics showed that the both Ruein and Azotobarvar biofertilizers were in desirable condition and Phosco biofertilizer, despite having some growth-promoting properties, lacked a sufficient microbial population and the Potabarvar biofertilizer lacked the microbial population.

Keywords

References

Adesemoye AO and Kloepper JW, 2009. Plant microbe's interactions in enhanced fertilizer-use efficiency. Applied Microbiology and Biotechnology 85: 1–12.
Bashan Y and de-Bashan LE, 2010. How the plant growth-promoting bacterium Azospirillum promotes plant growth–a critical assessment. Advances in Agronomy 108: 77–136.
Benizri E, Courtade A, picard C and Guckert A, 1998. Role of Maize root exudates in the production of auxins by pseudomonas putida M6,1. Soil Biology and Biochemistry 30: 1481-1484.
Bric J, Bostock M and Silverstone SE, 1991. Rapid in situ assay for indole acetic acid production by bacteria immobilized on a nitrocellulose membrane. Applied and Environmental Microbiology 57:755 -760.
Deaker R, László Kecskés M, Timothy Rose M, Amprayn K, Krishnen G, ThiKimCuc T, Thuy Nga V, Thi Cong P, Thanh Hien N and Kennedy I, 2011. Practical methods for the quality control of inoculant biofertilizers. ACIAR (Australian Centre for International Agricultural Research).
Haggag WM, 2010. Role of entophytic microorganisms in biocontrol of plant diseases. Life Science Journal 7: 57- 62.
He Y, Oyaizu H and Suzuki S, 2002. Indole-3-acetic acid production in Pseudomonas fluorescens HP72 and its association with suppression of creeping bentgrass brown patch. Current Microbiology 47: 138–143.
Heidarpour A, Aliasgharzad N, Khoshmanzar E, Khoshru B and Asgari Lajayer B, 2019. Bio-removal of Zn from contaminated water by using green algae isolates. Environmental Technology & Innovation 16: 330-343.
Husen E, Simanungkalit RD, Saraswati R and Irawan J, 2007. Characterization and quality assessment of Indonesian commercial biofertilizers. Indonesian Journal of Agricultural Science 98: 31-38
Jeon JS, Lee SS, Kim HY, Ahn TS and Song HG, 2003. Plant growth promoting in soil by some inoculated microorganisms. Journal of Microbiology 41(4) 271- 276.
Kanimozhi K and Panneerselvam A, 2010. Studies on isolation and nitrogen fixation ability of Azospirillum spp. isolated from Thanjavur district. Der Chemica Sinica 1: 138-145.
Khoshmanzar E, Aliasgharzad N, Neyshabouri MR, Khoshru B, Arzanlou M and Asgari Lajayer B, 2019. Effects of Trichoderma isolates on tomato growth and inducing its tolerance to water-deficit stress. International Journal of Environmental Science and Technology 17(2):869-78.
Khoshru B, Aliasgharzad N, Jodmand A, 2019a. The Effect of pH adjustment of municipal compost on its enrichment with plant growth promoting bacterium "Enterobacter Cloacae. Journal of Soil Biology 7 (1): 103-112. (In Persian).
Khoshru B and Sarikhani MR, 2019b, Effect of phosphatic microbial fertilizers produced from phosphate solubilizing bacteria on phosphorus uptake and growth of maize. Iranian Journal of Soil Research 33 (1): 13-24. (In Persian).
Khoshru B, Sarikhani MR and Aliasgharzad N. 2019c, Inoculation effect of some phosphatic microbial fertilizers on nutritional indices of Zea mays L. Water and Soil Science-University of Tabriz 29 (2): 15-27. (In Persian).
Khoshru B and Sarikhani MR, 2018. Isolation and identification of temperature resistant phosphate solubilizing bacteria for use in phosphatic microbial fertilizer. Journal of Water and Soil 32 (1): 155-167. (In Persian).
Khoshru B, Sarikhani MR and Ebrahimi M, 2017a. Isolation of temperature resistant phosphate solubilizing bacteria for use in phosphatic microbial fertilizer. The 15th Congress of Soil Science. 6-8 September. Isfahan. Iran. (In Persian).
Khoshru B, Sarikhani MR and Aliasgharzad N, 2017b. Application and non-application of sulfur in the formulation of Pseudomonas fluorescens phosphatic microbial fertilizer on corn (Zea mays L.). Journal of Agricultural Sciences and Austainable Production-University of Tabriz 27(3):119-136. (In Persian).
Khoshru B, Sarikhani MR and Lotfollhi A, 2017c. Inoculation effect of some phosphatic microbial fertilizers prepared using thermal resistant PSB on Zea mays. The 15th Congress of Soil Science. 6-8 September. Isfahan. Iran. (In Persian).
Khoshru B, Sarikhani MR, Aliasgharzad N and Zare P, 2015a. Assessment the important PGPR features of isolates used in biofertilizers Barvar2, Biosuperphosphate, Supernitroplus and Nitroxin, Applied Soil Research 3(1): 39-52. (In Persian).
Khoshru B, Sarikhani MR and Aliasgharzad N, 2015b. Molecular and biochemical identification of the bacterial isolates used in common biofertilizers in Iran. Water and Soil Science-University of Tabriz 25(4.2): 13-26. (In Persian).
Kizilkaya R, 2009. Nitrogen fixation capacity of Azotobacter spp. strains isolated from soils in different ecosystems and relationship between them and the microbiological properties of soils. Journal of Environmental Biology 31: 73- 82.
Leylasi Marand M and Sarikhani MR. Evaluation of nitrogen fixing efficiency of some Azotobacter isolates in solid and liquid LG medium by Kjeldahl method. Water and Soil 28(2): 205-215. (In Persian)
Liu W, Xu X, Wu X, Yang Q, Luo Y and Christie P, 2006. Decomposition of silicate minerals by Bacillus mucilaginosus in liquid culture. Environmental Geochemistry and Health 28:133–140.
Motsara MR and Roy RN, 2008. Guide to Laboratory Establishment for Plant Nutrient Analysis. Food and Agriculture Organization of the United Nations. The University of Michigan, USA.
Rajai S, Alikhani HA and Raeisi F, 2007. Effect of plant growth promoting potentials of Azotobacter chroococcum native strains on growth, yield and uptake of nutrients in wheat. Science and Technology Journal of Agriculture and Natural Resources 41: 296-285.
Sarikhani MR and Ansari S, 2015. Evaluation of some qualitative characteristics of common biofertilizers in Iran. Sustainable Agriculture 1-14. (In Persian)
Sarikhani MR, 2016. Increasing potassium (K) release from K-containing minerals in the presence of insoluble phosphate by bacteria. Biological Journal of Microorganism 4: 87-96.
Sarikhani MR, Khoshru B and Greiner R. 2019. Isolation and identification of temperature tolerant phosphate solubilizing bacteria as a potential microbial fertilizer. World Journal of Microbiology and Biotechnology 35(8): 126-139.
Sarikhani MR, Aliasgharzad N and Khoshru B, 2018. Effectiveness study of phosphate solubilizing bacteria in the formulation of phosphatic microbial fertilizers on Corn. Iranian Journal of Soil and Water Research 3(1): 81-71. (In Persian).
Sarikhani MR, Khoshru B and Oustan S, 2016. Efficiency of some bacterial strains in potassium release from mica and phosphate solubilization under in-vitro conditions. Geomicrobiology Journal 33(9): 832-838.
Sperber JI, 1958. Solution of apatite by soil microorganisms producing organic acids. Australian Journal of Agricultural Research 9: 782-787.
Sugumaran P and Janarthanam B, 2007. Solubilization of potassium containing minerals by bacteria and their effect on plant growth. World Journal of Agriculture Sciences 3: 350-335.
Tamura K, Dudley J, Nei M and Kunars S, 2007. Molecular evolutionary genetics analysis (MEGA)
software version 4.0. Molecular Biology and Evolution 24(8):1596-9
Teaumroong N, Wanapu C, Chankum Y, Arjharn W, Sang-Arthit S, Teaimthaisong K and Boonkerd N, 2010. Production and Application of Bioorganic Fertilizers for Organic Farming Systems in Thailand. Microbes at Work, Springer, Berlin Heidelberg.
Vessey JK, 2003. Plant growth promoting rhizobacteria as biofertilizers. Plant and Soil 255: 571–586.
Whitelaw MA, Harden TJ and Helyar KR, 1999. Phosphate solubulisation in solution culture by the soil fungus Penicillium radicum. Soil Biology and Biochemistry 31: 655-665.
Widnyana IK, Suprapta DN, Sudana IM and Temaja IGRM, 2011. Pseudomonas alcaligenes, potential antagonist against Fusarium oxysporum f. sp lycopersicum the cause of fusarium wilt disease on tomato. Journal of Biology, Agriculture and Healthcare 3:163-169.
Water and Soil Science
Volume 30, Issue 3
September 2020
Pages 15-29

Files

Share

How to cite

Statistics