Screening and Identification of Heat Resistant Phosphate Solubilizing Bacteria

Document Type : Research Paper

Authors

1 PhD Student of Soil Biology and Biotechnology, Faculty of Agriculture, University of Tabriz, Iran

2 Assoc. Prof. of Soil Biology and Biotechnology, Faculty of Agriculture, University of Tabriz, Iran

Abstract

Bacteria with high phosphate solubility and resistance to high temperature are suitable candidates for use in granular phosphatic microbial fertilizers (PMF). The prepared product in processing of these fertilizers (PMF) is exposed to high temperatures (50-55 ° C) under very low moisture conditions. Accordingly, in this research, the efficiency of phosphate dissolution and temperature tolerance of 150 bacterial isolates were evaluated. These bacteria were prepared in the soil biology laboratory of University of Tabriz. The phosphate solubility of these bacteria was evaluated by semi-quantitative and quantitative methods in solid and liquid Sperber medium in the presence of low-soluble tricalcium phosphate (TCP) source at the temperatures of 28 °C and 55 °C. The results showed that there was a significant difference between bacteria in terms of dissolution of phosphate. At a typical temperature among 150 bacteria, 25 bacteria (17%) had no phosphorus dissolution capacity, and the HD/CD ratio for these 25 bacteria was 1.2 to 2.8. In the quantitative method, the solubility potential of 25 isolates was 175.88- 292.98 mg P/l. Among the 25 isolates, only 7 bacteria were able to survive at 55 ° C for 16 hours, witch between them the C1-4O, C19-4O and C8-12M bacteria had the highest halo diameters with values of 2.5, 2.4 and 2.2 for the HD/CD ratio, respectively. The amounts of phosphorus release by these bacteria in the liquid medium were 244.08, 256.44 and 216.14 mg/l, respectively. The molecular identification of these bacteria showed that they were belonged to the genus Enterobacter and Stenotrophomonas.

Keywords

References

Afzal A, Ashraf M, Asad SA and Farooq M, 2005. Effect of phosphate solubilizing microorganisms on phosphorus uptake, yield and yield traits of Wheat (Triticum aestivum L.) in rain fed area. International Journal of Agriculture and Biological Sciences 7: 2. 207-209.
Aliasgharzad N, 1997. Soil Microbiology and Biochemistry (translation). First Edition. Tabriz University Press.
Alikhani HA, Saleh-Rastin N and Antoun H, 2006. Phosphate solubilization activity of rhizobia native to Iranian soils. Plant  and Soil 287: 35-41.
Anonymous, 2015. Protocols for Registration of Fertilizers Material. Institute of Soil and Water Research. (In Persian)
Chabot R, Hani A and Cescas PM, 1996. Growth promotion of maize and lettuce by phosphate-solubilizing Rhizobium leguminosarum biovar. phaseoli. Plant and Soil 184: 311-21.
Ghasemi Y, Kianmehr M H, Mirzabe A H and Abooali B, 2013. The effect of rotational speed of the drum on physical properties of granulated compost fertilizer. Physicochemical Problems of Mineral Processing 49 (2):743-755
Goldstein AH, 1994. Involvement of the quinoprotein glucose dehydrogenase in the solubilization of exogenous phosphates by gram-negative bacteria. Pp. 197-203. In: Torriani-Gorini A, Yagil E, Silver S, (eds.) Phosphate in Microorganisms: Cellular and Molecular Biology. Washington, DC: ASM Press.
Heydarian Z and Sarikhani MR, 2011. Growth promoting bacteria (PGPR) a promising approach to sustainable agriculture. 1th Specialized Conference on Strategies for Achieving Sustainable Agriculture. 5-6th of June, Ahvaz, Iran. (In Persian)
Hicks GC, Mc Camy IW and Norton MM, 1977. Studies of fertilizer granulation at TVA. In: Proceedings of the second, International Symposium on Agglomeration, Atlanta, USA.
Illmer P and Schinner F, 1995. Solubilization of inorganic calcium phosphates. Soil Biology and Biochemistry 46: 257-263.
Jeon JS, Lee SS, Kim HY, Ahn TS and Song HG, 2003. Plant growth promoting in soil by some inoculated microorganisms. Journal of Microbiology 271- 276.
Karpagam T and Nagalakshmi PK, 2014. Isolation and characterization of phosphate solubilizing microbes from agricultural soil. International Journal of Current Microbiology and Applied Sciences 3(3): 601-614.
Khan MS, Zaidi A and Wani PA, 2007. Role of phosphate-solubilizing microorganisms in sustainable agriculture-a review. Agronomy for Sustainable Development 27: 29-43
Khoshmanzar E, Aliasgharzad N, Neyshabouri MR, Khoshru B, Arzanlou M and Lajayer BA, 2019. Effects of Trichoderma isolates on tomato growth and inducing its tolerance to water-deficit stress. International Journal of Environmental Science and Technology, 1-10.
Khoshru B, Aliasgharzad N, Jodmand A, 2019a. The effect of pH adjustment of municipal compost on its enrichment with plant growth promoting bacterium "Enterobacter Cloacae". Journal of Soil Biology, 7 (1): 103-112. (In Persian).
Khoshru B and Sarikhani MR and Aliasgharzad N, 2019b. Inoculation effect of some phosphatic microbial fertilizers on nutritional indices of Zea mays L. Water and Soil Science, 29 (2): 15-27. (In Persian).
Khoshru B and Sarikhani MR, 2019. Effect of phosphatic microbial fertilizers produced from phosphate solubilizing bacteria on phosphorus uptake and growth of Maize. Iranian Journal of Soil Research, 33 (1): 13-24. (In Persian).
Khoshru B and Sarikhani MR, 2018. Isolation of temperature resistant phosphate solubilizing bacteria for use in phosphatic microbial fertilizer. Journal of Soil and Water 32(1): 155-167. (In Persian)
Khoshru B, Sarikhani MR, Aliasgharzad N and Zare P, 2015a. Assessment the important PGPR features of isolates used in biofertilizers Barvar2, Biosuperphosphate, Supernitroplus and Nitroxin. Applied Soil Research 3(1): 39-52. (In Persian)
Khoshru B, Sarikhani MR, and Aliasgharzad N, 2015b. Molecular and biochemical identification of the bacterial isolates used in common biofertilizers in Iran. Water and Soil Science, University of Tabriz 25 (4.2): 13-26. (In Persian)
Khoshru B, Sarikhani MR and Aliasgharzad N, 2017b. Application and Non-Application of Sulfur in the Formulation of Pseudomonas fluorescens Phosphatic Microbial Fertilizer on Corn (Zea mays L.). Journal of Agricultural Sciences and Sustainable Production 27(3):119-136. (In Persian)
Khoshru B. Sarikhani, MR and Ebrahimi M, 2017a. Isolation of temperature resistant phosphate solubilizing bacteria for use in phosphatic microbial fertilizer. The 15th Congress of Soil Science. 6-8 September. Isfahan. Iran. (In Persian)
Malboobi MA, Owlia P, Behbahani M, Sarokhani E, Moradi S, Yakhchali B, Deljou A and Morabbi Heravi K, 2009. Solubilization of organic and inorganic phosphates by three highly efficient soil bacterial isolates. World Journal of Microbiology and Biotechnology 25: 1471-1477
Motsara MR and Roy RN, 2008. Guide to Laboratory Establishment for Plant Nutrient Analysis. Rome: Food and Agriculture Organization of the United Nations; Oct 22.
Ostwal KP and Bhide VP, 1972. Solubilization of tricalcium phosphate by soil Pseudomonas. Indian Journal of Experimental Biology 10:153–4.
Pande A, Pandey P, Mehra S, Singh M and Kaushik S, 2017. Phenotypic and genotypic characterization of phosphate solubilizing bacteria and their efficiency on the growth of maize (Zea mays. L), International Journal of Agriculture Innovations and Research 5: 929-938
Pozin ME, 1986. Fertilizer Manufacture. Mir publishers. USSR. Moscow.
Reddy BC, Murthy D V S and Rao C D P, 1997. Modeling of a rotary drum granulator for control. Particle and Particle Systems Characterization 14: 257-262.
Rezaeifar J, 2008. Investigation parameters of pellets from cattle manure for extruder design. MSc Thesis. Aboureyhan College. University of Tehran, Tehran, Iran. (In Persian)
Sarikhani MR, Khoshru B and Oustan S, 2016. Efficiency of some bacterial strains in potassium release from mica and phosphate solubilization under in vitro conditions. Geomicrobiology Journal 33(9): 832-838.
Sarikhani MR, Oustan S, Ebrahimi M and Aliasgharzad N, 2018. Isolation and identification of potassium-releasing bacteria in soil and assessment of their ability to release potassium for plants. European Journal of Soil Science 69(6): 1078-1086.
Shakeela S, Padder SA and Bhat, ZA, 2017. Isolation and characterization of plant growth promoting rhizobacteria associated with medicinal plant Picrorhiza Kurroa. Journal of Pharmacognosy and Phytochemistry 6(3):157-168.
Sherrington PJ and Oliver R, 1981. Globulation Processes in Granulation. Heyden and Son Ltd., London.
Soltani Tolarod EA, Salehrastin N, Khavazi K, Asadi Rahmani H and Abbaszadeh P, 2008. Separating and study of plant growth promoting (PGP) in some Pseudomonas fluorescent native Iranian soil. Journal of Soil and Water Sciences 21(2): 278.
Son HJ, Park GT, Cha MS and Heo MS, 2006. Solubilization of insoluble inorganic phosphates by a novel-salt and pH-tolerant Pantoea agglomerans R-42 isolated from soybean rhizosphere. Bioresource Technology 97: 204-210.
Sperber J I, 1958. Solution of apatite by soil microorganisms producing organic acids. Australian Journal of Agricultural Research 9: 782-787.
Teaumroong N, Wanapu C, Chankum Y, Arjharn W, Sang-Arthit S, Teaimthaisong K and Boonkerd N, 2010. Production and Application of Bioorganic Fertilizers for Organic Farming Systems in Thailand. Microbes at Work, Springer, Berlin Heidelberg.
Yazdani M, Bahmanyar MA, Pirdashti H and Esmaili MA, 2009. Effect of phosphate solubilization microorganisms (PSM) and plant growth promoting rhizobacteria (PGPR) on yield and yield components of Corn (Zea mays L.). World Academy of Science, Engineering and Technology 37: 90-92.
Ziaeyan A, Salim-pour S, Silsipour M and Safari H, 2010. Evaluation of some bio and chemical P- fertilizers in corn. The 1st Iranian Fertilizer Challenges Congress Half a Century of the Fertilizer Consumption. 10-12 March, Tehran, Iran. (In Pesian)
Ghanbari, S. 2008. Investigation of influencing parameters on motion and stability angle granular materials in rotating cylinders. MSc Thesis Aboureyhan College University of Tehran, Tehran, Iran. (In Persian)
 
Water and Soil Science
Volume 30, Issue 2
June 2020
Pages 1-14

Files

Share

How to cite

Statistics