تأثیر روش کاربرد اسید هیومیک بر زیست‌فراهمی آهن در گیاه کلزا

نویسندگان

1 دانش آموخته کارشناسی ارشد علوم خاک دانشگاه علوم کشاورزی و منابع طبیعی گرگان

2 دانشیاران گروه علوم خاک دانشگاه علوم کشاورزی و منابع طبیعی گرگان

3 استادیار گروه علوم خاک دانشگاه علوم کشاورزی و منابع طبیعی گرگان

4 دانشجوی دکتری گروه علوم خاک دانشگاه علوم کشاورزی و منابع طبیعی گرگان

چکیده

برای بررسی تأثیر کاربرد خاکی، محلول­پاشی و مصرف همراه با آب آبیاری (کودآبیاری) اسید هیومیک بر فراهمی آهن در گیاه کلزا (Brassica napus L.)آزمایشی در قالب طرح کاملاً تصادفی با 10 تیمار و 4 تکرار به­اجرا درآمد که تیمارها شامل مصرف خاکی اسید هیومیک در سه سطح (1، 2 و 4 گرم بر کیلوگرم خاک)، محلول­پاشی اسید هیومیک در سه سطح (1/0، 2/0 و 4/0 درصد) و همراه با آب آبیاری در سه سطح (1000، 2000 و 4000 میلی‌گرم در لیتر) و تیمار شاهد (بدون اسید هیومیک) بود. نتایج نشان داد که بیشترین غلظت آهن کل در ساقه و آهن فعال به‌ترتیب با میانگین 85 و 9/44 میلی­گرم بر کیلوگرم مربوط به تیمار 2000 میلی­گرم بر لیتر مصرف اسید هیومیک همراه با آب آبیاری و کمترین مقدار به­ترتیب با میانگین 6/54 و 4/20 میلی­گرم بر کیلوگرم مربوط به تیمار شاهد بود. همچنین آهن قابل استخراج خاک گلدان­ها پس از برداشت، توسط عصاره­گیرهای مختلف به­ترتیب اگزالات آمونیوم سریع> هیدروکسیل آمین هیدروکلراید  AC-EDTA< DTPA< AB-DTPA < EDTA< کاهش یافت. براساس نتایج به­دست آمده آهن استخراج شده به­وسیله بیشتر عصاره­گیرها با شاخص کلروفیل همبستگی معنی­دار داشتند. از بین عصاره­گیرها تنها آهن استخراج شده به وسیله DTPA با آهن کل ساقه (*69/0r=) و آهن فعال (*79/0r=) معنی­دار شدند و بین آهن عصاره­گیری شده توسط این عصاره­گیر و غلظت کل آهن برگ همبستگی ضعیف و غیرمعنی­دار (ns35/0r=) مشاهده گردید. در این تحقیق مشاهده شد اندازه­گیری آهن فعال می­تواند یک روش مناسب برای تشخیص کمبود آهن در گیاه کلزا باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Humic Acid Application Method on Bioavailability of Iron in Canola

نویسندگان [English]

  • T Nazari 1
  • M Barani Motlagh 2
  • E Dordipour 2
  • R Ghorbani Nasrabadi 3
  • S Sefidgar Shahkolaie 4
1 M.Sc. Graduate, Dept. of Soil Science, Gorgan Uni. of Agri. Sci.and Natural Res. Iran.
2 Assoc. Prof, Dept. of Soil Science, Gorgan Uni. of Agri. Sci.and Natural Res. Iran.
3 Assist. Prof, Dept. of Soil Science, Gorgan Uni. of Agri. Sci.and Natural Res. Iran.
4 Phd. Students, Dept. of Soil Science, Gorgan Uni. of Agri. Sci.and Natural Res. Iran.
چکیده [English]

In order to investigate the effect of acid humic application method including soil application, spraying and fertigation on iron (Fe) supplying in rapeseed, an expriment was caried out consisting of 10 treatments and 4 replications. Treatments included humic acid soil application in three levels (1, 2 and 4 g kg-1 soil), spraying at three levels (0.1, 0.2 and 0.4%) and with fertigation at three levels (1000, 2000 and 4000 mg L-1) and control treatment (without humic acid). The results showed that the highest total iron and active iron concentration in stalk were 85 and 44.9 mg kg-1, respectively, in the treatment of 2000 mg L-1 of humic acid with fertigation, and the lowest value, respectively, with an average of 54.62 and 20.40 mg kg-1 for control treatment. Also, the order of significance for extractable- Fe from the pot soil by various extractants was as follows: rapid ammonium oxalate > hydroxylamine hydrochloride>AC-EDTA> DTPA> AB-DTPA >EDTA. The results obtained in the present study showed that extracted iron was significantly correlated with chlorophyll content by most of the extractants. Among extractants, only extracted Fe with DTPA correlated positively and significantly with total Fe (r=0.69*) and active Fe (r=0.79*) while, total iron of leaf had weak and non-signifcant correlation (r= 0.35) with DTPA. Based on the results of this study, active iron measurement can be a suitable method for predicting iron deficiency in canola plants.

کلیدواژه‌ها [English]

  • Active iron
  • Available iron
  • Canola
  • Extractants
  • Humic acid
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